{"product_id":"shiitake-75-lentinus-edodes-mushroom-culture","title":"Shiitake 75 (Lentinus edodes) Mushroom Plate Culture","description":"\u003ch2\u003eShiitake 75 Mushroom Plate Culture\u003c\/h2\u003e\n\u003cp\u003eShiitake 75 (Lentinus edodes) Mushroom Plate Culture.\u003c\/p\u003e\n\u003cp\u003eEach culture plate is a 100x15mm petri dish inoculated with fresh Shiitake 75 (Lentinus edodes) mycelium growing on malt extract agar (MEA), and will arrive actively colonizing.\u003c\/p\u003e\n\u003ch3\u003eShiitake 75 Mushroom Plate Culture Lab Notes\u003c\/h3\u003e\n\u003cp\u003eAt Out-Grow's mycology lab, we have observed the following traits of this mushroom plate culture:\u003c\/p\u003e\n\u003cp\u003eShiitake 75 (Lentinula edodes) mycelium appears dense white to off-white on culture media, with a cottony to tufted texture that becomes more compact and leathery with age, often producing rhizomorphic strands from the inoculation point. As a strain of L. edodes, it shares the species' optimal growth temperature near 239°F on PDA, with radial extension declining above or below that range. Strain-specific sectoring may be observed; PDA at 239°F works well for consistent strain comparisons.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Use\u003c\/h3\u003e\n\u003cp\u003eMushroom plate cultures, typically on MEA culture plates, are used to grow and preserve mycelium. In most cases, sterile transfers of agar wedges from this plate are used to inoculate fresh culture media or sterilized grain spawn.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Storage\u003c\/h3\u003e\n\u003cp\u003eYour mushroom plate culture can be wrapped in parafilm and stored at normal room temps, in a sealed baggie, for up to 6 months. Out-Grow recommends you replate your mushroom plate culture every 6 months to ensure longevity of your purchase.\u003c\/p\u003e\n\u003cp\u003eTransfer your Shiitake 75 mycelium to fresh MEA every 3–6 months to keep your culture healthy and vigorous.\u003c\/p\u003e\n\n\n\u003ch3\u003eMushroom Plate Cultures Are Made to Order\u003c\/h3\u003e\n\u003cp\u003eAll mushroom culture plates are made to order. Once your order is received, Out-Grow will inoculate your plate within 1-3 days. Your mushroom plate culture will then be incubated in our mycology lab for approximately 7-14 days, or until clean healthy mycelium has been verified by our mycologist. Once verified, your order will be shipped promptly. Please allow an additional week for your mushroom plate culture to fully colonize upon arrival, as colonization time varies by species.\u003c\/p\u003e\n\u003cdiv class=\"og-internal-links\" style=\"margin-top:16px;padding:16px;background:#f7f4ef;border-top:2px solid #c8d4c4;font-family:'Source Serif 4',Georgia,serif;\"\u003e\n\u003ch3 style=\"font-family:'Libre Baskerville',serif;color:#1a2e1a;margin:0 0 10px;font-size:1.1rem;\"\u003eRelated Resources\u003c\/h3\u003e\n\u003cul style=\"list-style:none;padding:0;margin:0;display:grid;grid-template-columns:1fr 1fr;gap:6px 16px;\"\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/shiitake-75-lentinus-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake 75 (Lentinus edodes) Species Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/how-to-grow-shiitake-75-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake 75 (Lentinus edodes) Grow Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/products\/shiitake-75-lentinus-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake 75 (Lentinus edodes) Liquid Culture\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Out-Grow","offers":[{"title":"Default Title","offer_id":50046518034625,"sku":null,"price":24.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0772\/7287\/5201\/files\/Mushroom_Culture_Plate.png?v=1773334304","url":"https:\/\/www.out-grow.com\/products\/shiitake-75-lentinus-edodes-mushroom-culture","provider":"Out Grow","version":"1.0","type":"link"}