{"product_id":"shiitake-cw-40-60-fahrenheit-lentinula-edodes-mushroom-culture","title":"Shiitake CW 40-60 fahrenheit (Lentinula edodes) Mushroom Plate Culture","description":"\u003ch2\u003eShiitake CW 40-60 fahrenheit Mushroom Plate Culture\u003c\/h2\u003e\n\u003cp\u003eShiitake CW 40-60 fahrenheit (Lentinula edodes) Mushroom Plate Culture.\u003c\/p\u003e\n\u003cp\u003eEach culture plate is a 100x15mm petri dish inoculated with fresh Shiitake CW 40-60 fahrenheit (Lentinula edodes) mycelium growing on malt extract agar (MEA), and will arrive actively colonizing.\u003c\/p\u003e\n\u003ch3\u003eShiitake CW 40-60 fahrenheit Mushroom Plate Culture Lab Notes\u003c\/h3\u003e\n\u003cp\u003eAt Out-Grow's mycology lab, we have observed the following traits of this mushroom plate culture:\u003c\/p\u003e\n\u003cp\u003eShiitake CW (Lentinula edodes) mycelium appears dense white to off-white on culture media, with a cottony to tufted texture that becomes more compact and leathery with age. As a cold weather strain selected for fruiting at 40-60°F (39–59°F), it may show reduced radial growth at the species-typical optimal of 239°F compared to warm-weather strains — performance at cooler incubation temperatures may be comparatively stronger. Rhizomorphic strand development is typical of the species. Use PDA for consistent culture work and expect strain-specific variation in growth rate and morphology.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Use\u003c\/h3\u003e\n\u003cp\u003eMushroom plate cultures, typically on MEA culture plates, are used to grow and preserve mycelium. In most cases, sterile transfers of agar wedges from this plate are used to inoculate fresh culture media or sterilized grain spawn.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Storage\u003c\/h3\u003e\n\u003cp\u003eYour mushroom plate culture can be wrapped in parafilm and stored at normal room temps, in a sealed baggie, for up to 6 months. Out-Grow recommends you replate your mushroom plate culture every 6 months to ensure longevity of your purchase.\u003c\/p\u003e\n\u003cp\u003eStore fully colonized plates at 35–41°F in darkness, sealed to prevent desiccation. Cold weather strains are well-suited to refrigerated storage; plan to transfer every 3-6 months.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Cultures Are Made to Order\u003c\/h3\u003e\n\u003cp\u003eAll mushroom culture plates are made to order. Once your order is received, Out-Grow will inoculate your plate within 1-3 days. Your mushroom plate culture will then be incubated in our mycology lab for approximately 7-14 days, or until clean healthy mycelium has been verified by our mycologist. Once verified, your order will be shipped promptly. Please allow an additional week for your mushroom plate culture to fully colonize upon arrival, as colonization time varies by species.\u003c\/p\u003e\n\u003cdiv class=\"og-internal-links\" style=\"margin-top:16px;padding:16px;background:#f7f4ef;border-top:2px solid #c8d4c4;font-family:'Source Serif 4',Georgia,serif;\"\u003e\n\u003ch3 style=\"font-family:'Libre Baskerville',serif;color:#1a2e1a;margin:0 0 10px;font-size:1.1rem;\"\u003eRelated Resources\u003c\/h3\u003e\n\u003cul style=\"list-style:none;padding:0;margin:0;display:grid;grid-template-columns:1fr 1fr;gap:6px 16px;\"\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/shiitake-cw-40-60-f-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake CW 40-60 fahrenheit (Lentinula edodes) Species Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/how-to-grow-shiitake-cw-40-60f-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake CW 40-60 fahrenheit (Lentinula edodes) Grow Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/products\/shiitake-cw-40-60-fahrenheit-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake CW 40-60 fahrenheit (Lentinula edodes) Liquid Culture\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Out-Grow","offers":[{"title":"Default Title","offer_id":50046518067393,"sku":null,"price":24.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0772\/7287\/5201\/files\/Mushroom_Culture_Plate.png?v=1773334304","url":"https:\/\/www.out-grow.com\/products\/shiitake-cw-40-60-fahrenheit-lentinula-edodes-mushroom-culture","provider":"Out Grow","version":"1.0","type":"link"}