{"product_id":"shiitake-mushroom-lentinula-edodes-mushroom-culture","title":"Shiitake Mushroom (Lentinula edodes) Mushroom Plate Culture","description":"\u003ch2\u003eShiitake Mushroom Plate Culture\u003c\/h2\u003e\n\u003cp\u003eShiitake Mushroom (Lentinula edodes) Mushroom Plate Culture.\u003c\/p\u003e\n\u003cp\u003eEach culture plate is a 100x15mm petri dish inoculated with fresh Shiitake Mushroom (Lentinula edodes) mycelium growing on malt extract agar (MEA), and will arrive actively colonizing.\u003c\/p\u003e\n\u003ch3\u003eShiitake Mushroom Plate Culture Lab Notes\u003c\/h3\u003e\n\u003cp\u003eAt Out-Grow's mycology lab, we have observed the following traits of this mushroom plate culture:\u003c\/p\u003e\n\u003cp\u003eShiitake (Lentinula edodes) mycelium appears dense white to off-white on culture media, with a cottony to moderately tufted texture that becomes more compact, leathery, and slightly brownish in older central regions with age. Prominent rhizomorphic strands radiate from the inoculation point on well-colonized plates. Growth is vigorous, with one study reporting approximately 7mm per day radial extension on PDA at the optimal temperature of 239°F — growth declines noticeably above or below this range. Strains vary considerably and may exhibit sectoring; plate morphology is commonly used to informally screen strains for spawn performance. PDA at 239°F works well for consistent comparative work, as growth rate varies significantly across different media formulations.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Use\u003c\/h3\u003e\n\u003cp\u003eMushroom plate cultures, typically on MEA culture plates, are used to grow and preserve mycelium. In most cases, sterile transfers of agar wedges from this plate are used to inoculate fresh culture media or sterilized grain spawn.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Storage\u003c\/h3\u003e\n\u003cp\u003eYour mushroom plate culture can be wrapped in parafilm and stored at normal room temps, in a sealed baggie, for up to 6 months. Out-Grow recommends you replate your mushroom plate culture every 6 months to ensure longevity of your purchase.\u003c\/p\u003e\n\u003cp\u003eTransfer your Shiitake mycelium to fresh MEA every 3–6 months to keep your culture healthy and vigorous.\u003c\/p\u003e\n\n\n\u003ch3\u003eMushroom Plate Cultures Are Made to Order\u003c\/h3\u003e\n\u003cp\u003eAll mushroom culture plates are made to order. Once your order is received, Out-Grow will inoculate your plate within 1-3 days. Your mushroom plate culture will then be incubated in our mycology lab for approximately 7-14 days, or until clean healthy mycelium has been verified by our mycologist. Once verified, your order will be shipped promptly. Please allow an additional week for your mushroom plate culture to fully colonize upon arrival, as colonization time varies by species.\u003c\/p\u003e\n\u003cdiv class=\"og-internal-links\" style=\"margin-top:16px;padding:16px;background:#f7f4ef;border-top:2px solid #c8d4c4;font-family:'Source Serif 4',Georgia,serif;\"\u003e\n\u003ch3 style=\"font-family:'Libre Baskerville',serif;color:#1a2e1a;margin:0 0 10px;font-size:1.1rem;\"\u003eRelated Resources\u003c\/h3\u003e\n\u003cul style=\"list-style:none;padding:0;margin:0;display:grid;grid-template-columns:1fr 1fr;gap:6px 16px;\"\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/how-to-grow-shiitake-wr-50-75f-mushrooms-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake Mushroom (Lentinula edodes) Grow Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/products\/shiitake-lentinula-edodes\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eShiitake Mushroom (Lentinula edodes) Liquid Culture\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Out-Grow","offers":[{"title":"Default Title","offer_id":50046517969089,"sku":null,"price":25.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0772\/7287\/5201\/files\/Mushroom_Culture_Plate.png?v=1773334304","url":"https:\/\/www.out-grow.com\/products\/shiitake-mushroom-lentinula-edodes-mushroom-culture","provider":"Out Grow","version":"1.0","type":"link"}