{"product_id":"white-button-mushroom-agaricus-bisporus-mushroom-culture","title":"White Button Mushroom (Agaricus bisporus) Mushroom Plate Culture","description":"\u003ch2\u003eWhite Button Mushroom Plate Culture\u003c\/h2\u003e\n\u003cp\u003eWhite Button Mushroom (Agaricus bisporus) Mushroom Plate Culture.\u003c\/p\u003e\n\u003cp\u003eEach culture plate is a 100x15mm petri dish inoculated with fresh White Button Mushroom (Agaricus bisporus) mycelium growing on malt extract agar (MEA), and will arrive actively colonizing.\u003c\/p\u003e\n\u003ch3\u003eWhite Button Mushroom Plate Culture Lab Notes\u003c\/h3\u003e\n\u003cp\u003eAt Out-Grow's mycology lab, we have observed the following traits of this mushroom plate culture:\u003c\/p\u003e\n\u003cp\u003eWhite Button Mushroom (Agaricus bisporus) mycelium appears dense and white on PDA, with robust full-plate coverage under optimal conditions. Studies report that common commercial strains reach full 9cm plate coverage in 8-12 days at 77°F, corresponding to roughly 7.5-11mm per day radial growth — with 77°F identified as the optimal incubation temperature on agar, growth slowing noticeably at 68°F and 86°F and significantly at 59°F. This species shows sensitivity to medium pH, performing best at near-neutral to slightly alkaline conditions — pH 7-8 produces the most rapid and uniform growth, while pH 6 is notably slower. PDA at pH 7-8 and 77°F is the recommended baseline for lab culture and comparative assays.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Use\u003c\/h3\u003e\n\u003cp\u003eMushroom plate cultures, typically on MEA culture plates, are used to grow and preserve mycelium. In most cases, sterile transfers of agar wedges from this plate are used to inoculate fresh culture media or sterilized grain spawn.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Culture Storage\u003c\/h3\u003e\n\u003cp\u003eYour mushroom plate culture can be wrapped in parafilm and stored at normal room temps, in a sealed baggie, for up to 6 months. Out-Grow recommends you replate your mushroom plate culture every 6 months to ensure longevity of your purchase.\u003c\/p\u003e\n\u003cp\u003eStore fully colonized plates at 35–41°F in darkness, sealed to prevent desiccation. Working plates should be refreshed every 3-6 months to maintain culture vigor.\u003c\/p\u003e\n\u003ch3\u003eMushroom Plate Cultures Are Made to Order\u003c\/h3\u003e\n\u003cp\u003eAll mushroom culture plates are made to order. Once your order is received, Out-Grow will inoculate your plate within 1-3 days. Your mushroom plate culture will then be incubated in our mycology lab for approximately 7-14 days, or until clean healthy mycelium has been verified by our mycologist. Once verified, your order will be shipped promptly. Please allow an additional week for your mushroom plate culture to fully colonize upon arrival, as colonization time varies by species.\u003c\/p\u003e\n\u003cdiv class=\"og-internal-links\" style=\"margin-top:16px;padding:16px;background:#f7f4ef;border-top:2px solid #c8d4c4;font-family:'Source Serif 4',Georgia,serif;\"\u003e\n\u003ch3 style=\"font-family:'Libre Baskerville',serif;color:#1a2e1a;margin:0 0 10px;font-size:1.1rem;\"\u003eRelated Resources\u003c\/h3\u003e\n\u003cul style=\"list-style:none;padding:0;margin:0;display:grid;grid-template-columns:1fr 1fr;gap:6px 16px;\"\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/white-button-mushroom-agaricus-bisporus\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eWhite Button Mushroom (Agaricus bisporus) Species Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/pages\/how-to-grow-button-mushrooms-agaricus-bisporus\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eWhite Button Mushroom (Agaricus bisporus) Grow Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/products\/white-button-mushroom-agaricus-bisporus\" style=\"color:#4a6e42;text-decoration:underline;\"\u003eWhite Button Mushroom (Agaricus bisporus) Liquid Culture\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Out-Grow","offers":[{"title":"Default Title","offer_id":50046518984897,"sku":null,"price":24.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0772\/7287\/5201\/files\/Mushroom_Culture_Plate.png?v=1773334304","url":"https:\/\/www.out-grow.com\/products\/white-button-mushroom-agaricus-bisporus-mushroom-culture","provider":"Out Grow","version":"1.0","type":"link"}