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How Many Holes Does a Monotub Really Need and Can You Have Too Many?

How Many Holes Does a Monotub Really Need and Can You Have Too Many?

How Many Holes Does a Monotub Really Need and Can You Have Too Many?

The first monotub I ever drilled, I stood over a clear storage tote with a hole saw in my hand, second guessing every mark I had made. I went with six because that was the number that kept coming up, and it worked. For a standard 50 to 75 liter tub, about six 2 inch holes is the right place to start: two on each long side just above the substrate line, and one on each short end near the top. And yes, you can have too many, just not for the reason most beginners assume.

Too many holes becomes a problem when they are large or left wide open, because they dry the surface out faster than your mushrooms can pin. The hole count is a coarse setting. The real control is how you cover those holes.

Out-Grow Quick Answer

  • Start with about six 2 inch holes on a 50 to 75 liter tub: two per long side just above the substrate, one on each short end up high.
  • The number six is a heuristic, not a rule. Tune it to your tub size and how dry your room is.
  • Yes, you can have too many. Oversized or wide-open holes dry the surface, stall pins, and invite contamination.
  • Change the coverage before the count. Polyfill and tape adjust airflow far more precisely than drilling new holes.
  • Tape every hole shut during colonization, then open and stuff them for fruiting.
6
Holes to start with on a standard 50 to 75 liter tub
2in
Hole diameter most build guides land on, about 5 centimeters
50 to 75L
Tub volume the six-hole pattern is built around

How Many Holes Does a Monotub Need to Start With

Back to that first tub. Six 2 inch holes, two on each long side sitting just above where the substrate would come up to, and one on each short end as high as I could put it. That pattern has fruited more tubs for me than I can count, and it is the same layout most careful build guides converge on for a mid-size tote.

Here is why six works, and it has nothing to do with the number being magic. Two low inlets on each long side and one high outlet on each end give you a symmetric set of high and low openings, which lets air move gently through the tub while leaving most of the plastic shell intact to hold moisture. You get exchange without turning the tub into a colander. Treat six as your baseline, then read your own grow and adjust. A drier room may want a touch less open area, a very humid room can take a little more.

Fresh air exchangenoun

The rate at which stale, carbon dioxide rich air inside the tub is replaced with fresh air from the room. Mushrooms need it to form normal caps and stems. Your holes, the lid gap, and how you cover the holes together set the fresh air exchange, which is the single thing this whole build is trying to balance against humidity.

Where to Drill the Holes in a Monotub

A grower once sent me photos of a tub that kept weeping water out of its lower holes, and the fix was obvious the moment I saw it. The holes were drilled too low, right at the substrate surface, so every bit of condensation ran straight out. Placement matters as much as count, and it is easy to get wrong on the first build.

Put the two holes on each long side just above the substrate line, not down at it, so they sit in open air rather than in the wet substrate. Put the single hole on each short end up near the top edge. A figure you will see quoted is about 8 inches above the bottom of the bin, which works fine on a tall tote, but the honest version is that the right height depends on how deep your substrate sits, so aim for just above the surface rather than a fixed measurement.

Standard Monotub Hole Spec, 50 to 75 Liter Tub
Hole count
About 6 total
Hole diameter
2 inch, about 5 centimeters
Lower holes
2 per long side, just above the substrate
Upper holes
1 per short end, near the top edge
Substrate depth
5 to 10 centimeters, about 2 to 4 inches
Fruiting headspace
10 to 20 centimeters above the substrate
During colonization
All holes taped, lid gap only
During fruiting
Lower holes stuffed tight, upper loose

With the spec in hand, the drilling itself is the quick part. Mark your spots first, keep them even, and cut on a clear tote so light can reach the surface later.

Drilling evenly spaced 2 inch holes in the side of a clear monotub for a mushroom fruiting chamber

Can You Have Too Many Holes in a Monotub?

I watched a beginner talk himself into a dozen holes once, reasoning that if six was good then twelve had to be better. His surface went bone dry inside a couple of days, the pins that had started just shriveled, and he could not understand why more air had hurt. That is the trap with monotub holes, and the answer is yes, you can absolutely have too many.

The damage does not really come from the count on its own. It comes from too much open, unfiltered area. Every large hole you add lets moisture escape and dries the mycelium at the surface, and dry mycelium pins poorly and gets invaded more easily. What actually decides your fresh air exchange is not just how many holes you drilled, but how much of their area is open, which is why the same six holes can run bone dry or perfectly balanced depending on what is stuffed in them.

The Same Six Holes, Very Different Airflow
Relative fresh air exchange from one six-hole tub, set entirely by how the holes are covered. Higher bar means more air and more drying.
All holes taped
Very low
Lower tight, upper loose
Low
Light polyfill all round
Medium
Holes wide open
High
Extra or oversized holes
Highest
Avoid
Do not drill more holes the moment growth looks off. Nine times out of ten the answer is to change how the existing holes are covered, not to cut new ones you can never uncut.

Monotub Hole Count vs Hole Coverage: How to Dial It In

When I set up a tub now, I barely think about the hole count after the drill goes down. All the tuning happens with a bag of polyfill and a roll of tape. That is the mental shift that fixes most monotub problems: the count is the coarse setting you pick once, and the coverage is the fine dial you turn for the rest of the grow.

The sequence below is how I run it from a freshly drilled tub through a balanced fruiting surface. Notice that only the first step touches the holes themselves. Everything after is coverage and observation.

Dialing In a Monotub, Coverage Not Count
1
Drill six 2 inch holes
Two on each long side just above the substrate line, one on each short end near the top. Use a clear tote so light reaches the surface.
2
Tape every hole for colonization
Cover all six with non-breathable tape and let the lid gap alone handle exchange. This traps moisture while the mycelium runs.
3
Pull the tape at full colonization
Once the substrate is fully colonized and starting to pin, remove the tape so fresh air exchange can rise.
4
Stuff lower tight, upper loose
Pack the lower holes firmly with polyfill and the upper holes loosely, so air drifts up and through instead of blasting across the surface.
5
Read the surface
You want a glistening surface with fine beads of water, not pooling and not dry. Watch the stems and caps as the pins develop.
6
Adjust coverage, not count
Leggy and fuzzy means loosen the polyfill. Dry and cracking means tighten it. Reach for the drill only as a last resort.

Here is what that coverage actually looks like in the holes themselves, a firm plug of polyfill down low and a looser tuft up high.

Polyfill stuffed into monotub holes to tune fresh air exchange during fruiting

The do and do not list below boils that same logic down to what to reach for and what to skip.

Do this
  • Start with about six 2 inch holes on a 50 to 75 liter tub
  • Tape every hole shut during colonization
  • Change the polyfill and tape before you change the count
  • Read the surface and the stem shape, then adjust
Avoid this
  • Drilling more holes the moment mushrooms look leggy
  • Leaving every hole wide open in a dry room
  • Chasing one magic hole count for every tub size
  • Treating the build as set and forget

Do You Tape Monotub Holes During Colonization?

The first tub I ran without taping the holes taught me the lesson fast. I left them open the whole time, thinking the mycelium wanted air, and the top of my substrate dried into a crust before it ever finished colonizing. Yes, you tape the holes during colonization, and it is one of the highest leverage moves in the whole build.

During colonization you want moisture held in and carbon dioxide allowed to build, because elevated carbon dioxide actually helps the mycelium run across the substrate. Cover all the holes with non-breathable tape and rely on the small gap around the lid for the little exchange that is needed. Once the tub is fully colonized and pins are starting, you pull the tape and switch the holes over to their fruiting job. The stages are not the same, and the holes serve a different purpose in each.

Monotub with all holes taped over during colonization to hold moisture

The holes are not doing the same job from start to finish, which is easy to see when you lay the stages out in order.

How the Holes Change Job by Stage
1
Colonization
All holes taped, fresh air exchange very low
2
Pinning
Tape off, lower tight and upper loose
3
Full fruiting
Coverage tuned to the shape of the mushrooms

Why Are My Monotub Mushrooms Skinny? Too Little Fresh Air Exchange

A grower emailed me a photo of his first oyster flush and it looked like pale coral, all long thin stems and tiny curled caps reaching up toward the lid. He was ready to blame his culture. It was not the culture. That look is the classic signature of too little fresh air exchange and too much carbon dioxide trapped in the tub.

When exchange is too low, the mushrooms stretch. You get long, thin, leggy stems, fuzzy mycelium creeping up the base of those stems, caps that stay small or never open, and in a high fresh air exchange species like oyster mushrooms you often see coral-like branching. Sometimes there is a faint off smell from the stale air. The fix is more exchange, but reach for coverage first: loosen the polyfill, crack the lid slightly, or thin out what is stuffed in the upper holes. Only if you have opened everything up and it is still starved would I consider another hole.

Leggy monotub oyster mushrooms with long thin stems from too little fresh air exchange

Monotub Surface Too Dry? Too Many Holes and Too Much Fresh Air Exchange

The opposite failure showed up in my own lab one winter when the room air was dry as paper. My tub surface lost its shine, the caps on the pins started to crack, and a whole set of primordia aborted before they got anywhere. That is what too much fresh air exchange does, and it is exactly what an over-drilled or wide-open tub gives you in a dry room.

When exchange runs too high, the substrate surface dries out and loses its glisten, caps crack, pins abort, the whole block can start to shrink, and desiccated mycelium gets easier for mold to invade. The move is to cut the exchange down without touching the drill: tighten or add polyfill, tape a couple of holes partly shut, raise the humidity in the room, or set a tray of wet vermiculite in the tub to feed moisture back in. The table below lines up both failure modes against a healthy surface so you can read your own tub at a glance.

What you see Too little fresh air exchange Dialed in Too much fresh air exchange
Stems and caps Long, thin, leggy, branching Short, sturdy stems, open caps Cracked caps, uneven growth
Surface Fuzzy mycelium climbing the stems Glistening, fine beads of water Dry, dull, losing its sheen
Pins Few, stretched, slow Even, healthy pinset Aborting and shrinking
The fix Loosen polyfill or crack the lid Hold it steady Tighten polyfill or raise room humidity

That dried, dull surface is worth learning to spot early, because it is the first warning that the tub is breathing too hard.

Dried cracked monotub substrate surface from too much fresh air exchange and too many open holes

What Humidity Should a Monotub Room Be?

A grower once fogged his entire spare room up to 90 percent trying to protect a single tub, then wondered why water was running down the walls and his tape kept peeling off. He had the target on the wrong container. A properly tuned monotub makes most of its own humidity, so the room does not need to be a rainforest.

Inside the tub, during fruiting, you are aiming for the same band most gourmet species want, which is 85 to 95 percent relative humidity right at the surface. The room around it can sit far lower, somewhere around 40 to 70 percent is plenty, because the sealed-in moisture and the taped or stuffed holes hold the tub itself high. Soaking the whole room just gives you condensation, dripping, and a better home for contamination. Watch the surface, not a room hygrometer.

Room Humidity Around a Monotub
The tub holds its own moisture, so the room can run much drier than the tub
Room is fine here, 40 to 70%
20%60%100%
Comfortable room range, 40 to 70% Soaking wet, invites condensation
Tip
Chase the surface, not the room. A glistening substrate with tiny beads of water tells you the tub is right, no matter what the room hygrometer reads.

Do Monotub Holes Change With Tub Size?

Someone scaling up from a shoebox to a big 100 liter tote asked me if he should just keep the same six holes, and the answer is no, not exactly. Six holes is built around a mid-size tub, and the pattern does not stretch cleanly to every size.

Think about it as area rather than a headcount. A larger tub has more substrate surface to exchange air over, so it usually wants more open area, and a small tub wants less. There is no clean published rule for holes per liter, so the honest guidance is to scale roughly with the surface area and then, as always, tune with coverage once the tub is running. A tiny tub might do fine with four modest holes kept mostly stuffed, while a large one might need more openings but still spend most of its life partly covered.

1
One rule scales to every tub size. Set the hole count roughly to the surface area, then let polyfill and tape do the real tuning. Coverage travels across tub sizes in a way that a fixed hole count never will.

Common Bad Advice About Monotub Holes

The advice that costs beginners the most is the confident claim that there is one correct number of holes and that more of them is always better. I have watched that idea dry out more first grows than almost anything else. A common rule you will see is that more fresh air exchange is always better, and it simply is not true past a point, because too much airflow dries the surface and drives aborts. Here is the accurate version.

Monotub Hole Myths vs Facts
Myth

There is one correct number of holes, and more holes always means better airflow and bigger yields.

Fact

Six 2 inch holes is a starting pattern for a mid-size tub, not a law. Past a point, extra or oversized holes dry the surface and stall pinning. Coverage, not count, tunes the airflow.

Myth

Leave all the holes wide open during fruiting so the mushrooms can breathe.

Fact

In most rooms you stuff the holes with polyfill to slow the air down. Wide-open holes suit only a very humid room or a high fresh air exchange species.

Myth

Drill the holes exactly 4 inches from the bottom, every time.

Fact

The right height is relative to the substrate line, not a fixed measurement, because substrate depth and tub size vary. Aim the lower holes just above the surface.

Myth

Keep the room at 80 to 90 percent humidity or the tub will dry out.

Fact

A tuned tub holds its own humidity. The room can sit around 40 to 70 percent, and soaking it invites condensation and contamination.

Out-Grow Lab Note
In years of talking growers through a stalled monotub, the fix is almost never another hole. It is loosening or tightening the polyfill that is already in the holes, or moving the tub to a room with steadier humidity. The drill is the last tool I reach for, not the first.

Relevant Out-Grow Supplies

A monotub is one of the cheapest fruiting chambers there is, since the tub itself is a plastic tote from any hardware store, and we do not sell those. So this list is short and honest, just the consumables that actually go into tuning a tub, plus the simpler paths if a monotub is more than you want to manage.

  • Mushroom growing supplies. Polyfill, tape, and the small gear that does the real tuning on a monotub live here. This is the coverage side of the build, which matters more than the drill.
  • All in one grow bags. Honest fit: if drilling and dialing a tub sounds like a lot for a first grow, one of these fruits right in its own bag with no holes to balance at all.
  • Mushroom grow kits. The simplest path of all. A kit arrives colonized and ready to fruit, so you skip the chamber build entirely while you learn to read a surface.
  • The full mushroom supply catalog. Browse here for substrate, bags, and the rest of a fruiting setup once you have decided how you want to grow.

The Bottom Line on Monotub Holes

A monotub needs about six 2 inch holes on a standard 50 to 75 liter tub, and yes, you can have too many when they are large or left wide open and dry the surface out. The number is only a starting point. Tape every hole during colonization, open and stuff them for fruiting, and then tune the airflow with polyfill and tape instead of the drill. Get in the habit of reading the surface and the shape of your mushrooms, and the right amount of fresh air exchange stops being a guess.

About the author

Mike Wiberg is the founder of Out-Grow, a mushroom cultivation supply company he has run since 2009. He developed the original all in one mushroom grow bag in 2014, a format now used across the hobby, and over more than 20 years of hands-on mycology he has grown hundreds of species and researched substrate formulations from the lab bench. He maintains one of the largest commercial culture libraries online, including a working collection of more than 300 species under a USDA permit. His cultivation work has been cited in peer reviewed research published in Frontiers in Cellular and Infection Microbiology, and he is one of only a handful of growers with a documented successful clone of Matsutake, one of the most difficult species in cultivation. He has helped growers troubleshoot cultivation problems for nearly two decades, and he writes from the lab and the grow room, not from a keyword list.

Monotub Holes FAQ

How many holes does a monotub need?
About six 2 inch holes for a standard 50 to 75 liter tub: two on each long side just above the substrate line, and one on each short end near the top. Treat that as a starting point and tune with coverage from there.
Can you have too many holes in a monotub?
Yes. Too many large or wide-open holes dry the substrate surface, stall or abort pins, and raise contamination risk. If growth looks off, adjust how the existing holes are covered before cutting new ones.
What size should monotub holes be?
A 2 inch hole, about 5 centimeters, is the size most build guides land on. That diameter gives enough open area to tune with polyfill while still being easy to stuff mostly shut when you need less airflow.
Where do you drill the holes in a monotub?
Put two on each long side just above where the substrate sits, and one on each short end up near the top edge. The lower holes handle intake and the upper holes let stale air drift out.
Do you tape monotub holes during colonization?
Yes. Cover all the holes with non-breathable tape during colonization to hold moisture and let carbon dioxide build, relying on the lid gap for the little exchange needed. Remove the tape once the tub is fully colonized and pinning.
Why are my monotub mushrooms tall and skinny?
Long thin stems and small caps mean too little fresh air exchange and too much carbon dioxide. Loosen the polyfill, crack the lid slightly, or thin the upper holes before you consider drilling more.
What humidity should the room around a monotub be?
The tub holds its own humidity, so the room can sit around 40 to 70 percent. Inside the tub during fruiting you want 85 to 95 percent at the surface. Read the glistening surface rather than a room hygrometer.
Do bigger or smaller tubs need a different number of holes?
Yes, roughly. Scale the open area with the substrate surface, so a larger tub usually wants more holes and a small one fewer. There is no clean holes-per-liter rule, so set the count by size and then tune with coverage.