How to Grow Shiitake Mushrooms

shiitake mushrooms fruiting on a log

How to Grow Shiitake Mushrooms at Home: A Complete Guide to Shiitake Mushroom Production on Logs and Substrate

Shiitake mushrooms are one of the most popular gourmet mushrooms, well-known for their rich flavor and incredible health benefits. If you're looking to grow shiitake mushrooms at home, you're in the right place. This guide will teach you everything you need to know about cultivating shiitake mushrooms, whether you want to grow shiitake on logs or use a substrate-based method. By the end of this post, you'll have the tools and knowledge to start growing your own shiitake mushrooms at home and enjoy fresh mushrooms year-round. Let's dive into the exciting world of shiitake mushroom production!



Shiitake Mushroom Cultivation: Logs vs. Substrate

When it comes to shiitake cultivation, there are two primary methods to choose from: growing on shiitake mushroom logs or using a substrate such as sawdust. Both approaches are highly effective, but each has its unique advantages depending on your growing space and resources. Below, we'll cover both methods in detail, ensuring that you can confidently choose the right one for your situation.

Shiitake Mushroom Logs: The Traditional Method

Growing shiitake on logs is the traditional method, and it can yield several flushes of mushrooms over the course of many years. To start, you'll need fresh hardwood logs, with white oak being one of the best options for cultivating shiitake mushrooms at home. Logs should be cut when trees are dormant (typically late winter to early spring) and should be 3 to 8 inches in diameter. Each log can produce pounds of mushrooms for years, making this method highly rewarding for long-term growers.

After the logs are cut, they need to be "rested" for about 2-8 weeks to allow natural antifungal agents in the wood to degrade. Once the logs are ready, you'll proceed with the inoculation process by drilling holes in the logs and inserting shiitake mushroom spawn, either in the form of sawdust spawn or plug spawn.

Shiitake Mushroom Substrate: The Faster Indoor Option

If you don’t have access to logs or want a faster turnaround time, growing shiitake mushrooms on a supplemented sawdust substrate is an excellent alternative. The substrate method allows you to grow shiitake mushrooms indoors with much faster fruiting times compared to logs. This method uses grow bags filled with sterilized sawdust and bran, which serve as the perfect growing medium for shiitake mycelium to colonize.

The bags are inoculated with shiitake mushroom spawn and kept in a warm, dark environment for several weeks until the mycelium completely colonizes the substrate. Once the substrate has turned from white to brown, the block is ready for fruiting. Shiitake mushrooms can start fruiting within a few months when grown on substrate, providing a quicker harvest for indoor growers.

Step-by-Step Guide to Growing Shiitake Mushrooms on Logs

Step 1: Selecting and Preparing Your Logs

To grow shiitake mushrooms on logs, you'll need to start by choosing the right hardwood. White oak, sugar maple, and ironwood are excellent choices for shiitake production. Make sure to cut your logs when the trees are dormant, as this helps retain the natural sugars that shiitake mycelium needs for growth. Logs should be 3-8 inches in diameter and 36-40 inches in length.

After cutting the logs, store them in a shaded area to prevent them from drying out. Logs should be rested for 2-8 weeks before inoculation to allow the tree’s natural defenses to break down. Logs that are too dry will not be suitable for mushroom inoculation, so it's essential to maintain moisture during this period by storing the logs in a shaded, moist area.

Step 2: Inoculating Your Shiitake Logs

Once your logs are ready, it's time to inoculate them with shiitake mushroom spawn. The drill-and-fill method is the most common technique for inoculating logs. To do this, you'll need a drill fitted with a 5/16-inch drill bit (for plug spawn) or a 7/16-inch drill bit (for sawdust spawn). Drill holes in the logs in a diamond pattern, spacing the holes about 6 inches apart.

After drilling the holes, insert either plug spawn or sawdust spawn into the holes. Plug spawn can be gently tapped in with a hammer, while sawdust spawn should be packed into the holes using an inoculation tool. Once the spawn is in place, seal each hole with wax to protect the spawn from contamination and retain moisture. Beeswax, paraffin, or cheese wax are all suitable options for this step.

Step 3: Incubating and Caring for Your Shiitake Logs

Maintaining Moisture Levels

Once the logs are inoculated, it's time to focus on maintaining proper moisture levels during the incubation period. Logs should be stored in a shaded area with plenty of natural rainfall or occasional watering if necessary. Shiitake mycelium thrives in moist conditions, so aim to keep your logs receiving about 1 inch of water per week.

Stacking Your Logs for Incubation

There are several methods for stacking your shiitake logs during incubation, with the most popular being the A-frame stacking method. This method allows for good air circulation while keeping the logs off the ground. It also helps maintain moisture levels and makes it easier to manage your logs for shiitake mushroom production.

Incubation can take anywhere from 6 months to 2 years, depending on the size of your logs and the shiitake strain you're using. During this time, it's important to regularly monitor the logs to ensure they are not drying out and to check for signs of contamination.

Step 4: Protecting Your Logs from Pests and Contamination

During the incubation phase, it's essential to monitor your logs for pests and contamination. Rodents and insects can sometimes attack the logs, which may harm the mycelium's development. To minimize the risk, consider covering your logs with a mesh net or placing them in a more controlled environment like a shaded greenhouse.

  • Keep an eye on potential contamination from mold or bacteria. If you notice any unwanted growth on the logs, it’s essential to isolate the affected log and treat it by scraping off the contamination or applying a diluted bleach solution.

Step 5: Monitoring Incubation Progress

Incubation can take anywhere from 6 months to 2 years, depending on the size of your logs and the shiitake strain you're using. During this time, it's important to regularly monitor the logs to ensure they are not drying out and to check for signs of contamination. Proper air circulation and moisture control are essential for mycelium colonization.

  • Look for white or brownish mycelium growth at the ends of the logs or tiny mushroom primordia forming under the bark.

Step 6: Maintaining and Caring for Shiitake Logs

Once you’ve completed the inoculation process and your logs are stacked in a shaded area, the real waiting begins. The incubation phase for shiitake logs can last anywhere from 6 to 18 months, depending on the size of the logs, climate, and the shiitake strain used. During this time, it’s essential to maintain proper moisture levels in the logs to ensure that the mycelium continues to grow and develop.

Your logs will need regular care to produce mushrooms over the long term. The logs need to be watered or sprinkled with water during dry periods to ensure they don’t dry out. Aim for about 1 inch of water per week if there is no natural rainfall. You can monitor the moisture content by inspecting the ends of the logs — they should remain moist and spongy, not cracked or dry.

To encourage healthy shiitake growing, ensure that your logs are kept in an environment that avoids extremes. Too much direct sunlight or exposure to harsh wind can dry out the logs and reduce your yields.

Signs of Fully Colonized Logs

Once your logs are fully colonized, you may notice a few signs indicating that the shiitake mycelium has successfully spread through the wood. The bark may feel slightly elastic and spongy, and you may see white or brownish mycelium at the ends of the logs. In some cases, tiny mushrooms might begin to pop out on their own, especially after periods of rain or increased humidity.

If your logs are in good condition and you’ve maintained the moisture levels properly, they will be ready for the next phase: fruiting. If the logs do not begin to produce mushrooms naturally, you can initiate fruiting through soaking or shocking the logs, especially after the logs have had time to incubate fully.

Step 7: Forcing Fruiting to Trigger Mushroom Production

If your logs haven’t fruited naturally or if you want to control the timing of your harvest, you can force fruiting by soaking the logs in cold water. This step mimics the natural conditions that trigger shiitake mushrooms to fruit. Submerge the shiitake log in clean, cold water (preferably 20°F colder than the air temperature) for 12-24 hours.

Logs can be soaked in large tubs, stock tanks, or even clean ponds. After soaking, place the logs upright in a shaded, humid environment to encourage fruiting. Within a few days, you should see the first flush of mushrooms emerging.

This process of soaking and fruiting can be repeated every 8-12 weeks, as long as the logs remain healthy. The first flush tends to be the most abundant, but logs can produce multiple flushes over several years, with each subsequent flush yielding slightly fewer mushrooms.

Step 8: Harvesting Your Shiitake Mushrooms

Once your shiitake mushrooms grow, it’s important to know when and how to harvest them to get the best results. The best time to harvest your shiitake mushrooms is when the caps are about 50-75% open and still slightly curled at the edges. Mushrooms with fully opened, flat caps are typically past their prime and will not store as well.

To harvest, simply twist the mushroom gently at the base and pull it from the log. You can also use a sharp knife or scissors to cut the mushrooms from the log. Avoid handling the caps too much, as this can bruise them and reduce their shelf life. Place your freshly picked shiitake mushrooms in a breathable container, such as a basket or paper bag, and store them in the refrigerator if not using them immediately.

Post-Harvest Care and Future Flushes

After the first flush of mushrooms, give the logs time to rest for a few months before forcing another flush. During this resting period, continue to keep the logs moist and store them in a shaded area. Healthy logs can produce mushrooms for 4-6 years, with multiple flushes each growing season.

Over time, the yield will decrease, but logs that are well cared for can continue producing fresh shiitake mushrooms for years. With proper moisture management, some logs can produce as many as 3-4 flushes per year. The logs will naturally decay over time, eventually breaking down completely, at which point they can be composted or returned to the environment to continue the natural cycle of decomposition.

Step 9: Troubleshooting Common Issues with Shiitake Logs

While growing shiitake on logs is a fairly straightforward process, a few common issues can arise:

  • Mushrooms Don’t Appear: If your logs aren’t fruiting, they may be too dry, or the incubation period may not be complete. Make sure the logs are kept moist and consider shocking them with cold water to trigger fruiting.
  • Small or Deformed Mushrooms: This is often caused by insufficient moisture or lack of airflow. Ensure the logs are well-hydrated, and that the growing environment is properly ventilated.
  • Log Decay: If your logs begin to rot or decay, it may be due to too much moisture or poor ventilation. Keep the logs off the ground, elevated on racks or pallets, and ensure proper air circulation.

By maintaining proper moisture levels and ensuring that your logs have access to both shade and humidity, you can maximize the life of your logs and continue enjoying fresh shiitake mushrooms for years to come.

How to Grow Shiitake Mushrooms Using Substrate

Growing shiitake mushrooms on a prepared substrate allows for faster growth cycles and is ideal for indoor cultivation. Whether you're a beginner or an experienced mushroom grower, this method will help you successfully grow shiitake mushrooms without needing a forest or large outdoor space.

Step 1: Making Your Shiitake Mushroom Spawn

Before starting, you need to create or buy shiitake spawn, which is the "seed" that will colonize your substrate. If you're not using pre-made spawn, here's how to make your own mushroom spawn from scratch:

What You’ll Need to Make Shiitake Spawn

  • Grain (wheat, rye, or millet)
  • A pressure cooker that can reach 15 PSI
  • Mason jars with filter lids or autoclavable spawn bags
  • A shiitake mushroom culture or spores (either liquid culture or a colonized agar plate)
  • A sterile environment for working with mycelium (e.g., a still air box or laminar flow hood)

Preparing Grain Spawn

Grain is used as a base to grow your shiitake mycelium before it is transferred to the substrate. Here's how to prepare it:

  1. Hydrate the grain: Place your grain (e.g., rye or wheat) in a large pot and soak it in water for 24 hours. This helps the grain absorb moisture for mycelial growth.
  2. Simmer the grain: After soaking, bring the pot to a simmer for 15-20 minutes to soften the grain. The grain should be soft but not mushy, and the water should be absorbed.
  3. Drain and dry: Pour the grain into a strainer and let it drain for about 30 minutes. The grain should feel moist but not waterlogged. To test, squeeze a few grains in your hand; they should not break apart or be too sticky.
  4. Load jars or bags: Place the hydrated grain into Mason jars or spawn bags. Make sure the jars are only filled two-thirds of the way to allow room for shaking later.
  5. Sterilize: Put the jars or bags into a pressure cooker. Sterilize at 15 PSI for 90 minutes to 2 hours to kill any contaminants.

Inoculating the Grain

Once the grain has cooled, it’s time to inoculate it with your shiitake mushroom culture or spores:

  1. Clean your work area: You’ll need a sterile environment to inoculate the grain. Use a still air box or laminar flow hood and wipe everything down with 70% alcohol.
  2. Inoculate the jars or bags: Using a sterilized syringe, inject 1-2 ml of liquid shiitake culture into the jar, or, if you’re using agar, transfer small pieces of mycelium into the grain using a sterilized scalpel.
  3. Seal and incubate: Once inoculated, seal the jars or bags and store them in a warm, dark area (70°F-75°F). Shake the jars every few days to ensure even distribution of the mycelium.

In about 2-3 weeks, your grain will be fully colonized with shiitake mycelium and ready to be transferred to the prepared substrate. The grains should be fully white and covered with mycelium.

Step 2: Preparing Your Shiitake Substrate

Shiitake mushrooms grow well on hardwood-based substrates. A common recipe for substrate includes hardwood sawdust and a nutrient supplement like wheat or rice bran. Here's how to prepare the substrate properly:

What You’ll Need for the Substrate

  • Hardwood sawdust (oak, maple, or beech)
  • Wheat or rice bran (to supplement nutrition)
  • Water
  • Grow bags with filter patches or plastic containers
  • A pressure cooker

Substrate Recipe

  • 2 parts hardwood sawdust (aged, not fresh wood)
  • 1 part wheat or rice bran
  • Water (about 60-65% moisture level)

Mix these ingredients thoroughly in a large container. The moisture content is crucial—use sawdust that isn’t overly dry, and add enough water so that the mixture holds together when squeezed but doesn’t release more than a few drops of water.

Sterilizing the Substrate

  1. Load the bags: Fill your grow bags with the prepared substrate, but leave some space at the top for expansion.
  2. Seal the bags: Seal the grow bags either with an impulse sealer or by folding the top and securing it with clips.
  3. Sterilize: Place the grow bags into a pressure cooker and sterilize at 15 PSI for 90 minutes to 2 hours.

Once sterilized, allow the bags to cool completely before moving on to inoculation.

Step 3: Inoculating the Substrate

With the substrate prepared and sterilized, it’s now time to introduce the shiitake spawn into the bags. This process, known as inoculation, is critical for ensuring successful colonization.

Inoculation Process

  1. Work in a sterile environment: Sterility is key to preventing contamination. Prepare a still air box or laminar flow hood, and disinfect all surfaces.
  2. Break up the spawn: If you're using grain spawn, break it into smaller pieces inside the jar or bag to make sure it can be distributed evenly.
  3. Inoculate the substrate: Open the cooled, sterilized substrate bags and add the grain spawn (about 10% of the substrate weight). Gently shake or massage the bag to distribute the spawn evenly throughout the substrate.
  4. Seal the bags: Once inoculated, reseal the bags and place them in a warm, dark area (around 70°F).

The mycelium will begin to spread throughout the substrate, and the bags should start showing signs of colonization within a couple of weeks.

Step 4: Incubation of the Substrate

Once inoculated, the substrate bags will enter the incubation phase. During this stage, the shiitake mycelium will grow and colonize the entire substrate, eventually preparing the block for fruiting.

Incubation Conditions

  • Temperature: Keep the bags at around 70°F-75°F (21°C-24°C) in a dark environment.
  • Humidity: The environment should be slightly humid, but the bags should not be exposed to direct moisture during this stage.
  • Time: Incubation can take 8-12 weeks. You’ll notice the substrate turning white as the mycelium colonizes it. The block will eventually start to turn brown, indicating it’s getting close to fruiting.

Once the entire substrate block is brown and solid, it is ready to enter the fruiting stage.


Step 5: Fruiting Your Shiitake Mushrooms

To initiate the fruiting process, you’ll need to "shock" the fully colonized substrate block and provide it with the right environmental conditions.

Shocking the Substrate

  1. Soak the substrate block: Once fully colonized, remove the block from the grow bag and submerge it in cold water (50-55°F) for 12-24 hours. This mimics the environmental changes that trigger fruiting in nature.
  2. Place in a fruiting chamber: After soaking, place the substrate block in a high-humidity fruiting chamber with indirect light.

Fruiting Conditions

  • Temperature: 60-68°F (16-20°C)
  • Humidity: Keep the chamber at 85-90% humidity, misting the block regularly.
  • Light: Indirect natural light or low-intensity artificial light.

Within a few days, you should see the first mushrooms pop out of the substrate. Shiitake mushrooms tend to grow more slowly than some other species like oyster mushrooms, but with proper care, your block can produce mushrooms for several flushes over the course of months.

Step 6: Shiitake Mushroom Fruiting and Development

Once the substrate block has been shocked and placed in a suitable fruiting chamber, the fruiting process begins. This is the stage where your hard work pays off and shiitake mushrooms grow from the colonized substrate.

Creating the Ideal Fruiting Conditions

The environment for fruiting should closely mimic the natural conditions shiitake mushrooms experience in the wild. Shiitake mushrooms need a combination of high humidity, fresh air, and indirect light to produce optimal results:

  • Humidity: Maintaining 85-90% humidity is crucial. Mist the substrate block regularly to ensure it stays moist, but avoid direct soaking, as this can lead to contamination.
  • Temperature: The best temperature for fruiting shiitake mushrooms is 60-68°F (16-20°C), which helps to produce mushrooms at a consistent rate.
  • Airflow: Although shiitake mushrooms can tolerate higher levels of CO2, providing sufficient fresh air helps prevent deformed mushrooms and supports their development.
  • Light: Indirect natural light or low-intensity artificial light (around 200 lux) should be provided. You should be able to comfortably read in the light level that the mushrooms are exposed to.

Mushroom Development Stages

After a few days of exposure to these ideal conditions, you’ll start to see small pins or “little mushrooms” appearing on the substrate block. These are the early stages of mushroom development. As the mushrooms continue to grow, they will expand over the next 7-10 days, eventually reaching maturity.

It's important to monitor your growing conditions carefully to ensure the mushrooms develop correctly. If the environment is too dry or lacks airflow, your mushrooms may fail to reach full size or develop poorly.

Signs the Substrate is Ready to Fruit

When the substrate is fully colonized, it will start to turn brown and form bumps across the surface, which is often referred to as "pop-corning." This is an indicator that the block is ready to fruit. After shocking the block, pins will appear within a few days, and the mushrooms will be ready to harvest in another week or so.

Step 7: Harvesting Shiitake Mushrooms

Knowing the right time to harvest your shiitake mushrooms is crucial for getting the best flavor and texture. Harvest too early, and the mushrooms will be underdeveloped. Wait too long, and they may lose their firmness and become less desirable.

When to Harvest

You should harvest your shiitake mushrooms when the caps have expanded, but before they completely flatten out. At this stage, the gills under the mushroom cap will still be slightly covered, and the edge of the cap will have a slight curl. The mushrooms should be firm and have a robust color.

How to Harvest Shiitake Mushrooms

  1. Harvesting Method: To harvest, simply grasp the base of the mushroom near the substrate and twist gently. Alternatively, you can use a knife or scissors to cut the stem close to the block. Avoid pulling too hard, as this may damage the substrate or nearby developing mushrooms.
  2. Regular Harvesting: It’s essential to check your fruiting chamber daily, as mushroom growth can be rapid once they reach maturity. Harvest regularly to prevent over-mature mushrooms, which have reduced shelf life.
  3. Storing Fresh Mushrooms: After harvesting, store your shiitake mushrooms in a breathable container (such as a paper bag) in the refrigerator. They can be kept fresh for up to two weeks, but their flavor is best within the first week.

Maximizing Multiple Flushes

One of the advantages of growing shiitake mushrooms on substrate is that the block can produce several flushes of mushrooms over time. After the first flush, the block will need a rest period before fruiting again.

  • Resting Period: After harvesting, allow the block to rest for 2-3 weeks in the fruiting chamber, maintaining slightly lower humidity and less frequent misting.
  • Second Flush: To initiate another flush, repeat the soaking process by submerging the block in cold water for 12-24 hours, then place it back in the fruiting chamber. The second flush may not be as large as the first, but it can still produce mushrooms for several cycles.

The substrate can continue to fruit for up to six months, producing 3-5 flushes before it becomes depleted of nutrients. At that point, you can compost the spent substrate or add it to your garden for a nutrient boost.

Conclusion: Growing Shiitake Mushrooms at Home

Whether you’re a seasoned mushroom grower or a beginner, growing shiitake mushrooms at home using the substrate method is an effective way to produce healthy, delicious mushrooms year-round. By following this detailed guide, you’ll be able to grow mushrooms on a small scale in your own space, ensuring a continuous supply of fresh shiitake mushrooms for months to come.

From preparing your own spawn to mastering fruiting conditions, the process can seem complex at first, but with practice, it becomes a rewarding and straightforward task. Start growing your shiitake mushrooms today and enjoy the satisfaction of homegrown produce in your own backyard or indoor setup!

Q: What materials do I need to start growing shiitake mushrooms?

A: To start growing shiitake mushrooms, you will need fresh hardwood logs, mushroom plug spawn or sawdust spawn, a drill to make holes in the logs, and wax to seal the inoculation points. You may also consider using grow bags if you prefer not to use logs.


Q: Can I use sawdust to grow shiitake mushrooms?

A: Yes, you can use sawdust as a substrate for growing shiitake mushrooms. Sawdust spawn is a popular option for inoculating logs or for use in grow bags.


Q: How do I inoculate the logs for growing shiitake mushrooms?

A: To inoculate the logs, drill holes into the log surface, insert the mushroom plug spawn or sawdust spawn into the holes, and then seal the holes with wax. This helps protect the spawn and gives it the best chance to colonize the logs.


Q: When are the shiitake logs ready to fruit?

A: Shiitake logs are ready to fruit typically 6-12 months after inoculation, depending on the conditions and the type of mushroom strain used. You will see mature mushrooms starting to appear on the log surface.


Q: How often can mushrooms be harvested from the logs?

A: Once the shiitake logs are ready to fruit, mushrooms can be harvested every few weeks during the growing season. Logs can continue to produce mushrooms for several years.


Q: What should I do if I have tried growing oyster mushrooms before?

A: If you have tried growing oyster mushrooms before, you already have some experience with mushroom cultivation. You can use similar techniques but adapt them to the specific needs of shiitake mushrooms, such as using hardwood logs and inoculating with shiitake-specific spawn.


Q: Can I grow shiitake mushrooms in my own backyard?

A: Yes, you can grow shiitake mushrooms in your own backyard. Choose a shaded area with good air circulation to place your inoculated logs. This provides a suitable environment for the shiitake mushrooms to grow.


Q: Are there commercial shiitake mushroom kits available?

A: Yes, there are commercial shiitake mushroom kits available. These kits typically include everything you need to start growing shiitake mushrooms at home, including pre-inoculated logs or sawdust blocks.


Q: What are the benefits of using different shiitake mushroom strains?

A: Different shiitake mushroom strains can offer various benefits, such as different fruiting times, yield sizes, and resistance to different environmental conditions. Choosing the right strain can help you optimize your mushroom production based on your specific growing conditions.


Q: How do I know when the mushrooms are ready to be harvested?

A: Shiitake mushrooms are ready to be harvested when the caps have fully opened and the edges start to flatten out. At this stage, the mushrooms are mature and have the best texture and flavor for consumption.