For your convenience Out-Grow has made an all in one brown rice flour and vermiculite based mushroom grow bag substrate to simplify the mushroom cultivation process. This all in one grow bag has different layers of substrates so there is no need to transfer substrates to different bags or trays thus reducing the chance of contamination. This all in one grow bag is brown rice flour based and works for most gourmet mushroom species. Fully hydrated and sterilized with a five micron filter patch and self healing injection port. Simply inoculate the rye berry layer of the substrate with 2-5 cc of your favorite gourmet mushroom spore solution. After inoculation allow your all in one bag to colonize in room temps for 4-6 weeks until your first flush of mushrooms. Mixing the bag once after 50% colonization is optional and can increase colonization times. These bags hold three pounds (about four quarts of substrate) which is much more substrate than the competition offers. Shiitake, Reshi, Oyster mushrooms and many more gourmet mushrooms will thrive off of this substrate. This substrate can be fruited in the bag however for best results remove the fully colonized substrate and place in a fruiting chamber once you start to see pinning.