Culture media faq
Potato Dextrose Agar (PDA)
Product Description: Potato Dextrose Agar (PDA) is a versatile and nutrient-rich medium designed for the optimal growth and isolation of mushroom mycelium. This easy-to-prepare mix ensures clear and consistent microbial growth, making it an essential tool for maintaining a contamination-free cultivation environment.
Specifications:
• Contains 114 grams (1/4 lb) of dry potato dextrose agar mix per container
• Easily prepared by mixing 20 grams with 500 ml of water to create 20 petri dishes (100 x 15 mm)
• Heat, stir, sterilize, and pour for a simple and straightforward preparation process
Preparation Instructions:
1. In a clean, heat-resistant container, combine 20 grams of PDA mix with 500 ml of distilled water.
2. Heat the mixture while stirring until it reaches a gentle boil and the PDA is fully dissolved.
3. Sterilize the solution by autoclaving at 15 psi for 15-20 minutes.
4. Allow the solution to cool to approximately 50-60°C (122-140°F) before pouring into sterile petri
dishes.
5. Let the agar solidify at room temperature before using or storing.
Storage and Shelf Life:
• Store the dry PDA mix in a cool, dry place away from direct sunlight and moisture.
• Properly stored, the dry mix has a shelf life of up to 2 years.
• Once prepared, store the petri dishes containing PDA at 2-8°C (36-46°F) for up to 1 month.
Key Benefits:
• Optimized for mushroom mycelium expansion and isolation
• Ensures clear and consistent microbial growth
• Easy to prepare with a simple and straightforward process
• Essential for maintaining a contamination-free cultivation environment
Recommended Uses:
• Isolation and purification of mushroom cultures
• Mycelial growth and expansion
• Strain storage and maintenance
• Contamination testing and identification
Quality and Safety:
• High-quality mix ensuring optimal fungal growth and spore germination
• Manufactured in a clean, controlled environment to maintain purity and consistency
• Gamma-irradiated or autoclaved to ensure sterility
Tips for Success:
• Always use sterile techniques when preparing and pouring PDA to minimize contamination risk.
• Ensure the PDA solution is well-mixed and fully dissolved before sterilizing.
• Allow the sterilized PDA to cool sufficiently before pouring to avoid condensation on petri dish
lids.
• Label petri dishes with the date, strain, and any other relevant information for easy tracking and
organization.
Dehydrated Malt Extract Agar (MEA)
Product Description: Dehydrated Malt Extract Agar (MEA) is a nutrient-rich medium optimized for the growth and germination of fungal spores. This easy-to-use formula simplifies the agar preparation
process, ensuring a sterile environment for robust mycelium propagation.
Specifications:
• Contains 1/4 pound (113 grams) of dehydrated malt extract agar per package
• Easily prepared by adding the mix to simmering water and sterilizing
• Provides a reliable, high-quality ingredient for agar preparation
Preparation Instructions:
1. Bring 3.25 cups (780 ml) of distilled water to a gentle boil in a clean, heat-resistant container.
2. Slowly add 4.5 tablespoons (38 grams) of MEA powder while stirring continuously.
3. Reduce heat and simmer the mixture for 10 minutes or until the MEA is fully dissolved.
4. Pour the liquid MEA into a clean, heat-resistant container and sterilize by autoclaving at 15 psi
for 30 minutes.
5. Allow the sterilized MEA to cool for approximately 1 hour before removing it from the pressure
cooker.
6. Pour the cooled MEA into sterile petri dishes as needed, and allow the agar to solidify at room
temperature before use.
Storage and Shelf Life:
• Store the dehydrated MEA in a cool, dry place away from direct sunlight and moisture.
• When properly stored, the dehydrated MEA has a shelf life of up to 2 years.
• Once prepared, store the petri dishes containing MEA at 2-8°C (36-46°F) for up to 1 month.
Key Benefits:
• Optimized for fungal growth and spore germination
• Easy-to-use formula simplifies agar preparation
• Ensures a sterile environment for mycelium propagation
• High-quality ingredient for reliable agar preparation
Recommended Uses:
• Isolation and propagation of fungal cultures
• Spore germination and mycelial growth studies
• Strain storage and maintenance
• Contamination testing and identification
Quality and Safety:
• Manufactured using high-quality ingredients to ensure consistent results
• Produced in a clean, controlled environment to maintain purity and reliability
• Gamma-irradiated or autoclaved to ensure sterility
Tips for Success:
• Always use sterile techniques when preparing and handling MEA to minimize contamination risk.
• Ensure the MEA is fully dissolved and well-mixed before sterilizing.
• Allow the sterilized MEA to cool sufficiently before pouring to prevent condensation on petri dish
lids.
• Label petri dishes with the date, strain, and any other relevant information for easy tracking and
organization.
Liquid Culture Premix (LCP)
Product Description: Liquid Culture Premix (LCP) is a high-quality, nutrient-rich medium designed for optimal mycelium growth and expansion. This premix follows the same recipe used by Out-Grow for their trusted liquid culture production, ensuring consistent and reliable results for mushroom cultivators.
Specifications:
• Contains 114 grams (1/4 lb) of Liquid Culture Premix per container
• Easily prepared by mixing 1 teaspoon of the premix with 1000 ml of distilled water
• Provides a sterile, nutrient-rich environment for mycelium growth and expansion
Preparation Instructions:
1. In a clean, heat-resistant container, bring 1000 ml of distilled water to a simmer.
2. Add 1 teaspoon of the Liquid Culture Premix to the simmering water while stirring continuously
for 2-3 minutes.
3. Remove the mixture from heat and strain through a clean coffee filter to remove any
undissolved particles.
4. Pour the strained liquid into a clean, heat-resistant container and sterilize by autoclaving at 15
psi for 30 minutes.
5. Allow the sterilized liquid culture to cool to room temperature before inoculating with
mushroom cultures or storing.
Storage and Shelf Life:
• Store the dry Liquid Culture Premix in a cool, dry place away from direct sunlight and moisture.
• When properly stored, the dry premix has a shelf life of up to 2 years.
• Once prepared and sterilized, the liquid culture can be stored at room temperature for up to 6
months, provided it remains uncontaminated.
Key Benefits:
• High-quality premix optimized for mycelium growth and expansion
• Follows the same recipe used by Out-Grow for their trusted liquid culture production
• Easy preparation with distilled water for a hassle-free experience
• Ensures a sterile, nutrient-rich environment for successful mushroom cultivation
Recommended Uses:
• Expanding mycelium for inoculation of grain spawn or bulk substrates
• Propagating and storing mushroom cultures for long-term use
• Experimenting with different mushroom strains and varieties
Quality and Safety:
• Manufactured using high-quality ingredients to ensure consistent results
• Produced in a clean, controlled environment to maintain purity and reliability
• Gamma-irradiated or autoclaved to ensure sterility
Tips for Success:
• Always use sterile techniques when preparing and handling the liquid culture to minimize
contamination risk.
• Ensure the premix is fully dissolved and well-mixed before straining and sterilizing.
• Inoculate the cooled, sterilized liquid culture with a clean, viable mushroom culture to ensure
successful mycelium growth.
• Monitor the inoculated liquid culture for signs of contamination, such as off-colors or unusual
odors, and discard if necessary.
Agar - Agar Powder
Product Description: Agar - Agar Powder is a high-quality, versatile ingredient essential for preparing solid culture media in mushroom cultivation and mycology. This pure agar powder is optimized for fungal growth and provides a clear, stable substrate for mycelium propagation and isolation.
Specifications:
• Contains 1/4 pound (113 grams) of pure agar powder per package
• Easily incorporated into various agar media recipes for mushroom cultivation
• Provides a reliable, high-quality base for solid culture media preparation
Key Benefits:
• Optimized for mycology and fungal growth
• Essential for preparing agar media for petri dishes and slants
• Ensures a sterile, solid substrate for microbial cultivation
• Reusable and long-lasting when stored correctly
Recommended Uses:
• Preparation of various agar media, such as PDA, MEA, and custom blends
• Mycelium isolation, propagation, and strain storage
• Contamination testing and identification
• Spore germination and growth studies
Storage and Shelf Life:
• Store the agar powder in a cool, dry place away from direct sunlight and moisture.
• When properly stored, the agar powder has a shelf life of up to 3 years.
• Once prepared and sterilized, agar media should be stored at 2-8°C (36-46°F) for up to 1 month.
Quality and Safety:
• Manufactured using high-quality, pure ingredients to ensure consistent results
• Produced in a clean, controlled environment to maintain purity and reliability
• Gamma-irradiated or autoclaved to ensure sterility
Tips for Success:
• Always use sterile techniques when preparing and handling agar media to minimize
contamination risk.
• Follow precise measurements and recipes when incorporating agar powder into media blends.
• Ensure the agar is fully dissolved and well-mixed before sterilizing to achieve a homogeneous
substrate.
• Allow the sterilized agar media to cool sufficiently before pouring to prevent condensation on
petri dish lids or slant containers.
8 Ounces of Laboratory-Grade Dextrose
Product Description: 8 Ounces of Laboratory-Grade Dextrose is a high-quality, pure form of glucose essential for preparing nutrient-rich agar media and liquid cultures in mushroom cultivation. This dextrose provides a readily available energy source for fungal growth and evelopment, promoting robust mycelium propagation and healthy mushroom development.
Specifications:
• Contains 8 ounces (227 grams) of laboratory-grade dextrose per package
• Easily incorporated into various agar media and liquid culture recipes
• Provides a reliable, high-quality sugar source for mushroom cultivation
Key Benefits:
• High-quality, laboratory-grade dextrose ensures purity and consistency
• Essential for preparing nutrient-rich agar media and liquid cultures
• Supports robust mycelium propagation and healthy fungal development
• Versatile ingredient for various mushroom cultivation applications
Recommended Uses:
• Preparation of agar media, such as PDA and custom blends
• Formulation of liquid culture media for mycelium expansion
• Supplementation of grain spawn and bulk substrates
• Experimenting with different nutrient ratios for optimized mushroom growth
Storage and Shelf Life:
• Store the dextrose in a cool, dry place away from direct sunlight and moisture.
• When properly stored, the dextrose has a shelf life of up to 5 years.
• Once incorporated into media and sterilized, follow the storage guidelines for the specific media
type.
Quality and Safety:
• Manufactured using high-quality, pure ingredients to ensure consistent results
• Produced in a clean, controlled environment to maintain purity and reliability
• Laboratory-grade ensures minimal impurities and contaminants
Tips for Success:
• Always use sterile techniques when preparing and handling media containing dextrose to
minimize contamination risk.
• Follow precise measurements and recipes when incorporating dextrose into media blends.
• Ensure the dextrose is fully dissolved and well-mixed before sterilizing to achieve a
homogeneous solution.
• Experiment with different dextrose ratios and combinations with other nutrients to optimize
mushroom growth and yield.
8 Ounces of Light Malt Extract
Product Description: 8 Ounces of Light Malt Extract is a nutrient-dense, versatile ingredient used in the preparation of agar media and liquid cultures for mushroom cultivation. This high-quality malt extract provides a rich source of carbohydrates, proteins, and other essential nutrients that promote healthy mycelium growth and fungal development.
Specifications:
• Contains 8 ounces (227 grams) of light malt extract per package
• Easily incorporated into various agar media and liquid culture recipes
• Provides a reliable, nutrient-rich ingredient for mushroom cultivation
Key Benefits:
• Nutrient-dense composition optimized for fungal growth and mycelium propagation
• Essential for preparing high-quality agar media and liquid cultures
• Supports healthy mycelium development and robust mushroom growth
• Versatile ingredient for various mushroom cultivation applications
Recommended Uses:
• Preparation of malt extract agar (MEA) and other agar media blends
• Formulation of liquid culture media for mycelium expansion
• Supplementation of grain spawn and bulk substrates
• Experimenting with different nutrient ratios for optimized mushroom growth
Storage and Shelf Life:
• Store the light malt extract in a cool, dry place away from direct sunlight and moisture.
• When properly stored, the light malt extract has a shelf life of up to 2 years.
• Once incorporated into media and sterilized, follow the storage guidelines for the specific media
type.
Quality and Safety:
• Manufactured using high-quality ingredients to ensure consistent results
• Produced in a clean, controlled environment to maintain purity and reliability
• Tested for quality and purity to ensure suitability for mushroom cultivation
Tips for Success:
• Always use sterile techniques when preparing and handling media containing light malt extract
to minimize contamination risk.
• Follow precise measurements and recipes when incorporating light malt extract into media
blends.
• Ensure the light malt extract is fully dissolved and well-mixed before sterilizing to achieve a
homogeneous solution.
• Experiment with different light malt extract ratios and combinations with other nutrients to
optimize mushroom growth and yield.
Karo Syrup
Product Description: Karo Syrup is a high-quality, nutrient-rich ingredient used in the preparation of liquid culture media for mushroom cultivation. This pure, corn-based syrup provides a readily available source of carbohydrates and other essential nutrients that support healthy mycelium growth and development.
Specifications:
• High-quality, food-grade Karo Syrup
• Easily incorporated into liquid culture media recipes
• Provides a reliable, nutrient-rich ingredient for mushroom cultivation
Key Benefits:
• Nutrient-rich composition optimized for mycelium growth and development
• Essential for preparing high-quality liquid culture media
• Supports robust mycelium propagation and healthy mushroom growth
• Trusted by experienced mushroom cultivators for consistent results
Recommended Uses:
• Formulation of liquid culture media for mycelium expansion
• Supplementation of grain spawn and bulk substrates
• Experimenting with different nutrient ratios for optimized mushroom growth
• Use 1 tablespoon of Karo Syrup per 1 cup of water for optimal results
Preparation Instructions:
1. Combine 1 tablespoon of Karo Syrup with 1 cup of distilled water in a clean, heat-resistant
container.
2. Heat the mixture while stirring until the Karo Syrup is fully dissolved.
3. Pour the solution into your desired vessel and sterilize by pressure cooking at 12 psi for 20-30
minutes.
4. Allow the sterilized liquid culture media to cool to room temperature before inoculating with
mushroom cultures or storing.
Storage and Shelf Life:
• Store the Karo Syrup in a cool, dry place away from direct sunlight and moisture.
• When properly stored, unopened Karo Syrup has a shelf life of up to 2 years.
• Once incorporated into liquid culture media and sterilized, store the media at room temperature
and use within 1-2 months for best results.
Quality and Safety:
• Manufactured using high-quality, food-grade ingredients to ensure purity and consistency
• Trusted by experienced mushroom cultivators for reliable results
• Always use sterile techniques when preparing and handling liquid culture media to minimize
contamination risk
Tips for Success:
• Ensure the Karo Syrup is fully dissolved and well-mixed before sterilizing to achieve a
homogeneous solution.
• Experiment with different ratios of Karo Syrup to water to optimize mycelium growth for your
specific mushroom species.
• Monitor the inoculated liquid culture media for signs of contamination, such as off-colors or
unusual odors, and discard if necessary.
• Use high-quality, viable mushroom cultures to inoculate your liquid culture media for the best
results.
Sterilized Petri Dish 100 X 15mm (Pack of 20)
Product Description: Sterilized Petri Dish 100 X 15mm (Pack of 20) is a set of high-quality, pre-sterilized petri dishes essential for various mycology applications, including spore germination, mycelium isolation, and strain storage. These dishes provide a sterile environment for mushroom cultivation and are compatible with a wide range of agar media formulations.
Specifications:
• Contains 20 sterilized petri dishes per pack
• Dimensions: 100 mm diameter x 15 mm height
• Made from high-quality, clear polystyrene for easy observation
• Gamma-irradiated or autoclaved to ensure sterility
Key Benefits:
• Pre-sterilized and ready for immediate use, saving time and effort
• Optimized for fungal growth and mycelium propagation
• Ensures a sterile environment for successful mushroom cultivation
• Durable and stackable design for convenient storage and incubation
Recommended Uses:
• Pouring and solidifying agar media for mushroom cultivation
• Isolating and propagating pure mycelium cultures
• Storing and maintaining mushroom strains
• Conducting contamination tests and growth experiments
Quality and Safety:
• Manufactured using high-quality, food-grade polystyrene for clarity and durability
• Produced in a clean, controlled environment to maintain sterility
• Gamma-irradiated or autoclaved to eliminate contaminants
• Individually wrapped to preserve sterility until use
Tips for Success:
• Always use sterile techniques when handling petri dishes and pouring agar media to minimize
contamination risk.
• Allow the sterilized agar media to cool to approximately 50-60°C (122-140°F) before pouring to
prevent condensation on the petri dish lids.
• Avoid overfilling the petri dishes with agar media to prevent spillage and ensure proper lid
closure.
• Label the petri dishes with the date, media type, and any relevant strain information for easy
tracking and organization.
Dual-Sectioned Petri Dishes (100 x 15mm)
Product Description: Dual-Sectioned Petri Dishes (100 x 15mm) are innovative, high-quality petri dishes designed for efficient and space-saving mushroom cultivation experiments. Each dish features two compartments, allowing for side-by-side comparison of different strains, media formulations, or growth conditions.
Specifications:
• Contains 20 dual-sectioned petri dishes per pack
• Dimensions: 100 mm diameter x 15 mm height
• Made from high-quality, clear polystyrene for easy observation
• Two equal-sized compartments separated by a solid barrier
Key Benefits:
• Dual-sectioned design enables efficient side-by-side experiments and comparisons
• Saves space and resources by combining two tests in one petri dish
• High-quality polystyrene ensures clarity and durability
• Compatible with a wide range of agar media formulations
Recommended Uses:
• Comparing growth rates, morphology, or contamination resistance of different mushroom strains
• Testing the effects of various nutrients, supplements, or environmental factors on mycelium
growth
• Conducting antibiotic sensitivity or competition assays between mushroom species or
contaminants
• Optimizing agar media formulations for specific mushroom strains
Quality and Safety:
• Manufactured using high-quality, food-grade polystyrene for clarity and durability
• Produced in a clean, controlled environment to maintain purity and consistency
• Gamma-irradiated or autoclaved to ensure sterility (if specified as pre-sterilized)
• Individually wrapped (if pre-sterilized) to preserve sterility until use
Tips for Success:
• Always use sterile techniques when handling petri dishes and pouring agar media to minimize
contamination risk.
• Ensure equal distribution of agar media between the two compartments for accurate
comparisons.
• Label each compartment clearly with the relevant strain, media, or treatment information for
easy tracking and organization.
• If using non-sterile dishes, sterilize them before use by autoclaving or gamma-irradiation to
prevent contamination.
Triple-Sectioned 100 x 15mm Petri Dishes
Product Description: Triple-Sectioned 100 x 15mm Petri Dishes are innovative, high-quality petri dishes designed for efficient and space-saving mushroom cultivation experiments. Each dish features three compartments, allowing for side-by-side comparison of different strains, media formulations, or growth conditions.
Specifications:
• Contains 20 triple-sectioned petri dishes per pack
• Dimensions: 100 mm diameter x 15 mm height
• Made from high-quality, clear polystyrene for easy observation
• Three equal-sized compartments separated by solid barriers
• Pre-sterilized and ready for immediate use
Key Benefits:
• Triple-sectioned design enables efficient side-by-side experiments and comparisons
• Saves space and resources by combining three tests in one petri dish
• High-quality polystyrene ensures clarity and durability
• Pre-sterilized for immediate use, saving time and effort
Recommended Uses:
• Comparing growth rates, morphology, or contamination resistance of different mushroom strains
• Testing the effects of various nutrients, supplements, or environmental factors on mycelium
growth
• Conducting antibiotic sensitivity or competition assays between mushroom species or
contaminants
• Optimizing agar media formulations for specific mushroom strains
Quality and Safety:
• Manufactured using high-quality, food-grade polystyrene for clarity and durability
• Produced in a clean, controlled environment to maintain purity and consistency
• Gamma-irradiated or autoclaved to ensure sterility
• Individually wrapped to preserve sterility until use
Tips for Success:
• Always use sterile techniques when handling petri dishes and pouring agar media to minimize
contamination risk.
• Ensure equal distribution of agar media between the three compartments for accurate
comparisons.
• Label each compartment clearly with the relevant strain, media, or treatment information for
easy tracking and organization.
• Use the triple-sectioned petri dishes within the specified shelf life to ensure optimal
performance and sterility.
Four-Compartment Petri 100 x 15mm Petri Dishes
Product Description: Four-Compartment Petri 100 x 15mm Petri Dishes are innovative, high-quality petri dishes designed for efficient and space-saving mushroom cultivation experiments. Each dish features four compartments, allowing for side-by-side comparison of different strains, media formulations, or growth conditions.
Specifications:
• Contains 20 four-compartment petri dishes per pack
• Dimensions: 100 mm diameter x 15 mm height
• Made from high-quality, 100% virgin clear polystyrene for easy observation
• Four equal-sized compartments separated by solid barriers
• Sterilized and ready for immediate use
Key Benefits:
• Four-compartment design enables efficient side-by-side experiments and comparisons
• Saves space and resources by combining four tests in one petri dish
• High-quality, virgin polystyrene ensures clarity and durability
• Sterilized for immediate use, saving time and effort
Recommended Uses:
• Comparing growth rates, morphology, or contamination resistance of different mushroom strains
• Testing the effects of various nutrients, supplements, or environmental factors on mycelium
growth
• Conducting antibiotic sensitivity or competition assays between mushroom species or
contaminants
• Optimizing agar media formulations for specific mushroom strains
Quality and Safety:
• Manufactured using high-quality, 100% virgin clear polystyrene for clarity and durability
• Produced in a clean, controlled environment to maintain purity and consistency
• Sterilized to ensure a contamination-free environment for mushroom cultivation
• Single-use design prevents cross-contamination between experiments
Tips for Success:
• Always use sterile techniques when handling petri dishes and pouring agar media to minimize
contamination risk.
• Ensure equal distribution of agar media between the four compartments for accurate
comparisons.
• Label each compartment clearly with the relevant strain, media, or treatment information for
easy tracking and organization.
• Dispose of used petri dishes according to your laboratory's safety guidelines to maintain a clean
and safe work environment.
Culture Tube With Screw Cap (20 X 150 mm)
Product Description: Culture Tube With Screw Cap (20 X 150 mm) is a versatile and durable container designed for long-term storage of mushroom cultures and mycelium slants. The screw cap provides a secure, airtight seal, while the borosilicate glass construction ensures excellent clarity and resistance to thermal shock.
Specifications:
• Dimensions: 20 mm diameter x 150 mm height
• Material: Borosilicate glass for clarity and durability
• Screw cap with rubber liner for an airtight seal
• Sterilizable and reusable for multiple applications
Key Benefits:
• Ideal for long-term storage of mushroom cultures and mycelium slants
• Screw cap with rubber liner ensures a secure, airtight seal to prevent contamination
• Borosilicate glass construction provides excellent clarity and resistance to thermal shock
• Sterilizable and reusable design allows for multiple uses, reducing waste and costs
Recommended Uses:
• Long-term storage of mushroom cultures and mycelium slants
• Cultivation of liquid cultures for inoculation of grain spawn or bulk substrates
• Storage of stock cultures for future propagation or experimentation
• Isolation and purification of individual mushroom strains
Quality and Safety:
• Manufactured using high-quality borosilicate glass for clarity and durability
• Screw cap with rubber liner ensures a secure, airtight seal to maintain culture viability
• Can be sterilized using an autoclave or pressure cooker for reuse
• Gradual taper design minimizes the risk of tipping and spilling during handling
Tips for Success:
• Always use sterile techniques when handling culture tubes and preparing media to minimize
contamination risk.
• Ensure the screw cap is tightly sealed to maintain an airtight environment for long-term storage.
• Label the culture tube clearly with the strain, media, date, and any other relevant information
for easy tracking and organization.
• Store culture tubes in a cool, dark place to maintain culture viability and prevent unwanted
growth or contamination.
Liquid Culture Jar
Product Description: The Liquid Culture Jar is a pre-sterilized, ready-to-use container designed for the efficient expansion of mushroom mycelium in a liquid medium. This product includes a self-healing
injection port for easy inoculation and a gas exchange filter to maintain a sterile environment while
allowing for optimal mycelium growth.
Specifications:
• Sterilized liquid culture mix in a pint jar
• Self-healing injection port for easy inoculation
• Gas exchange filter for maintaining a sterile environment
• Converts 4 cc of spore solution into 100 cc of usable inoculate
Recommended Uses:
• Expanding mushroom mycelium from spore syringes or liquid culture syringes
• Creating a large volume of liquid culture inoculate for spawning grain or bulk substrates
• Storing and maintaining viable mycelium cultures for future use
Inoculation and Incubation:
1. Under sterile conditions, inject 2-4 cc of your chosen gourmet mushroom spore solution into the
jar through the self-healing injection port.
2. Gently swirl the jar to distribute the spore solution evenly throughout the liquid culture medium.
3. Place the inoculated jar in a clean, temperature-controlled environment between 70-80°F (21
27°C).
4. Monitor the jar for signs of mycelium growth, which typically appears within 7-14 days after
inoculation.
Harvesting and Use:
1. Once the mycelium has expanded to the desired amount, carefully remove the liquid culture
solution from the jar using a sterile syringe.
2. Use the harvested liquid culture to inoculate mushroom spawn bags, jars, or other prepared
substrates.
3. Repeat the process as needed to maintain a constant supply of viable liquid culture inoculate.
Key Benefits:
• Pre-sterilized and ready-to-use, saving time and effort in the inoculation process
• Self-healing injection port allows for easy, contamination-free inoculation
• Gas exchange filter maintains a sterile environment while promoting healthy mycelium growth
• Enables cost-effective expansion of mushroom cultures from a small volume of spore solution
Tips for Success:
• Always use sterile techniques when inoculating and handling the Liquid Culture Jar to minimize
contamination risk.
• Monitor the jar closely for any signs of contamination, such as off-colors or unusual odors, and
discard if necessary.
• Use high-quality, viable spore solutions or liquid cultures to ensure successful mycelium growth
and expansion.
Scalpel with 10 Sterile Blades
Product Description: The Scalpel with 10 Sterile Blades is a precision tool designed for a variety of
applications in mushroom cultivation and mycology. The set includes a durable, stainless steel scalpel
handle and 10 sterile, disposable carbon steel blades, ensuring a clean and precise cut every time.
Specifications:
• #3 stainless steel scalpel handle for durability and comfort
• 10 carbon steel scalpel blades, individually packaged and sterile
• Blade size: #10
• Sterilized by gamma irradiation
Key Benefits:
• Durable, ergonomic scalpel handle provides precise control and comfort during use
• Sterile, disposable blades ensure a clean cut and minimize contamination risk
• Ideal for a variety of mushroom cultivation tasks, including agar work and tissue transfers
• Individually packaged blades allow for easy blade changes and maintain sterility
Recommended Uses:
• Cutting and transferring mushroom tissue cultures on agar media
• Precision cutting of substrates, such as cardboard or wood, for inoculation
• Dissecting and examining mushroom fruiting bodies for research or cloning purposes
• General laboratory tasks requiring a clean, precise cutting tool
Quality and Safety:
• Scalpel handle made from high-quality, durable stainless steel
• Blades made from sharp, durable carbon steel for precise cuts
• Blades are sterilized by gamma irradiation to ensure a contamination-free tool
• Dispose of used blades in a proper sharps container to prevent accidents and contamination
Tips for Success:
• Always use sterile techniques when handling the scalpel and blades to minimize contamination
risk.
• Wear protective gloves and safety glasses when using the scalpel to prevent injuries.
• Change blades regularly to maintain sharpness and ensure clean, precise cuts.
• Store unused blades in their original packaging to maintain sterility until ready for use.
Magnetic Stir Bar (oval shape 7 count)
Product Description: The Magnetic Stir Bar set includes 7 high-quality, PTFE-coated stir bars in various
sizes. These stir bars are designed for use with magnetic stirrers to create a homogeneous mixture in
liquid culture media, chemical solutions, and other applications in mushroom cultivation and mycology
research.
Specifications:
• Material: PTFE (polytetrafluoroethylene) coating with a permanent magnet core
• Shape: Oval
• Quantity: 7 stir bars of different sizes
• Sizes: 1cm x 0.6cm, 1.5cm x 0.6cm, 2cm x 0.8cm, 2.5cm x 0.9cm, 3cm x 1cm, 3.5cm x 1.2cm, 4cm
x 1.5cm
Key Benefits:
• PTFE coating provides excellent chemical resistance and durability
• Permanent magnet core ensures a strong, stable magnetic coupling with the stirrer
• Various sizes accommodate different container volumes and stirring requirements
• Ideal for mixing liquid culture media, chemical solutions, and other liquids in mushroom
cultivation and research
Recommended Uses:
• Stirring and mixing liquid culture media for mushroom mycelium expansion
• Creating homogeneous solutions for agar media preparation
• Mixing chemical solutions or reagents for mycology research
• Stirring and dissolving substrates or supplements in liquid form
Quality and Safety:
• Made from high-quality, durable PTFE and permanent magnet materials
• PTFE coating is chemically inert and resistant to corrosion
• Withstands high temperatures and sterilization processes
• Smooth surface allows for easy cleaning and minimizes contamination risk
Tips for Success:
• Choose the appropriate stir bar size based on the container volume and stirring requirements.
• Ensure the stir bar is clean and sterile before use to prevent contamination.
• Use a magnetic stirrer with sufficient power to create a strong, stable vortex for efficient mixing.
• Store stir bars in a clean, dry place when not in use to maintain their quality and functionality.
Magnetic Stir Bar (cylinder shape 7 count)
Product Description: The Magnetic Stir Bar set includes 7 high-quality, PTFE-coated cylindrical stir bars in various sizes. These stir bars are designed for use with magnetic stirrers to create a homogeneous mixture in liquid culture media, chemical solutions, and other applications in mushroom cultivation and mycology research.
Specifications:
• Material: PTFE (polytetrafluoroethylene) coating with a permanent magnet core
• Shape: Cylindrical
• Quantity: 7 stir bars of different sizes
• Sizes: 3mm x 6mm, 4mm x 10mm, 7mm x 15mm, 7mm x 20mm, 7mm x 25mm, 6mm x 30mm, 8mm x 35mm
Key Benefits:
• PTFE coating provides excellent chemical resistance and durability
• Permanent magnet core ensures a strong, stable magnetic coupling with the stirrer
• Various sizes accommodate different container volumes and stirring requirements
• Cylindrical shape allows for efficient mixing and minimizes dead spots in the container
• Ideal for mixing liquid culture media, chemical solutions, and other liquids in mushroom
cultivation and research
Recommended Uses:
• Stirring and mixing liquid culture media for mushroom mycelium expansion
• Creating homogeneous solutions for agar media preparation
• Mixing chemical solutions or reagents for mycology research
• Stirring and dissolving substrates or supplements in liquid form
Quality and Safety:
• Made from high-quality, durable PTFE and permanent magnet materials
• PTFE coating is chemically inert and resistant to corrosion
• Withstands high temperatures and sterilization processes
• Smooth surface allows for easy cleaning and minimizes contamination risk
Tips for Success:
• Choose the appropriate stir bar size based on the container volume and stirring requirements.
• Ensure the stir bar is clean and sterile before use to prevent contamination.
• Use a magnetic stirrer with sufficient power to create a strong, stable vortex for efficient mixing.
• Store stir bars in a clean, dry place when not in use to maintain their quality and functionality.
Culture Test Tube Rack Holder
Product Description: The Culture Test Tube Rack Holder is a durable and versatile storage solution for organizing and holding test tubes used in mushroom cultivation and mycology research. Made from high-quality polypropylene, this rack can be heat sterilized without warping or odors, ensuring a clean and safe environment for your cultures.
Specifications:
• Material: Polypropylene
• Capacity: Holds up to 40 test tubes
• Tube diameter: Accommodates test tubes with a diameter of 20mm
• Dimensions: 245mm x 105mm (diameter) x 70mm (height)
• Assembly: Arrives in 3 pieces that snap together easily
Key Benefits:
• Durable polypropylene construction withstands repeated use and sterilization
• Heat sterilizable without warping or odors, ensuring a clean environment for cultures
• Holds up to 40 test tubes, providing ample storage space for your mushroom cultures
• Compatible with 20mm diameter test tubes, a standard size in mycology research
• Easy to assemble and disassemble for cleaning and storage
Recommended Uses:
• Organizing and storing test tube cultures for mushroom strain isolation and preservation
• Holding test tubes during liquid culture inoculation or transfer processes
• Keeping test tubes containing agar slants or other culture media organized and easily accessible
• Storing test tubes used for various mycology research applications
Quality and Safety:
• Made from high-quality, durable polypropylene that resists wear and tear
• Withstands repeated heat sterilization cycles without deformation or odors
• Smooth, non-porous surface allows for easy cleaning and minimizes contamination risk
• Sturdy construction ensures stability and prevents tipping or spilling of test tubes
Tips for Success:
• Ensure the rack and test tubes are clean and sterile before use to prevent contamination.
• Label test tubes clearly with the strain, media, date, and other relevant information for easy
tracking and organization.
• Use the rack in conjunction with other sterile techniques and equipment to maintain a clean,
contamination-free environment.
• Disassemble the rack periodically for thorough cleaning and sterilization to maintain a hygienic
workspace.