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Description

About This Mushroom Liquid Culture

Our Cold Weather 5 Pack is a premium mushroom liquid culture: live, lab-verified mycelium suspended in sterile nutrient broth and ready to inoculate your grow medium. Each set ships with sterile needles for clean, reliable transfers.

Cold Weather Liquid Culture 5 Pack — Product Summary

  • Five 10–12cc liquid culture syringes
  • Includes: White Enoki (Flammulina velutipes), King Oyster (Pleurotus eryngii), Pioppino (Agrocybe aegerita), Blue Oyster (Pleurotus columbinus), Chestnut (Pholiota adiposa)
  • Each syringe contains viable mycelium in sterile nutrient broth
  • Store liquid culture syringes at normal room temperature in the original packaging for 6 or more months.
  • Ships with sterile needles

Cold Weather Liquid Culture 5 Pack — Pack Overview

The Cold Weather Mushroom Cultures 5 Pack brings together five species that fruit reliably in cool to cold growing conditions, the species that keep producing when everything else in the grow room has stalled out. If you have a basement, garage, or grow space that stays cool in autumn and winter without supplemental heating, this pack was put together specifically for that situation. All five species have fruiting temperature ranges on the lower end of what we sell, and all five produce well at temperatures that most warm-weather species cannot tolerate.

Cold Weather Liquid Culture 5 Pack — Included Species

White Enoki (Flammulina velutipes) Liquid Culture

White Enoki is the star of this pack for cold-space cultivators, it fruits at 45 to 60°F and is one of very few cultivated species that actually performs better in cool conditions than at room temperature. If your grow space never gets warm enough for oyster mushrooms in winter, White Enoki is probably what you should be growing instead.

White Enoki — Out-Grow Lab Notes

Parameter Out-Grow Notes
Culture medium LME + dextrose, 2% + 2% in distilled water
Fruiting temp 45 to 60°F — the coldest-fruiting species in this pack
Substrate Hardwood sawdust; performs well in jars for stem elongation
Best for Spaces that never warm up in winter
Inoculation rate 2 to 3cc per quart of sterilized grain or hardwood substrate
Storage life 6 or more months at normal room temperature

White Enoki Taxonomy

  • Kingdom: Fungi
  • Division: Basidiomycota
  • Class: Agaricomycetes
  • Order: Agaricales
  • Family: Physalacriaceae
  • Genus: Flammulina
  • Species: Flammulina velutipes

King Oyster (Pleurotus eryngii) Liquid Culture

King Oyster is one of the cooler-fruiting Pleurotus species, performing well between 55 and 65°F. In a cool basement in autumn and early winter it fruits consistently when warmer oyster varieties are struggling. Restricting fresh air exchange during early pinning produces the characteristic long stem that makes it so distinctive.

King Oyster — Out-Grow Lab Notes

Parameter Out-Grow Notes
Culture medium LME + dextrose, 2% + 2% in distilled water
Fruiting temp 55 to 65°F
Key technique Restrict FAE during early pinning for stem elongation
Inoculation rate 2 to 3cc per quart of sterilized grain or hardwood substrate
Storage life 6 or more months at normal room temperature

King Oyster Taxonomy

  • Kingdom: Fungi
  • Division: Basidiomycota
  • Class: Agaricomycetes
  • Order: Agaricales
  • Family: Pleurotaceae
  • Genus: Pleurotus
  • Species: Pleurotus eryngii

Pioppino (Agrocybe aegerita) Liquid Culture

Pioppino performs well in the 55 to 75°F range and fruits through the cooler end of that window without the stalling that tropical species experience. The cluster-forming habit and nutty flavor make it a distinctive harvest, and it is a species that more cultivators working in cool spaces should try.

Pioppino — Out-Grow Lab Notes

Parameter Out-Grow Notes
Culture medium LME + dextrose, 2% + 2% in distilled water
Fruiting temp 55 to 75°F — comfortable at cool end of range
Substrate Supplemented hardwood sawdust
Inoculation rate 2 to 3cc per quart of sterilized grain or hardwood substrate
Storage life 6 or more months at normal room temperature

Pioppino Taxonomy

  • Kingdom: Fungi
  • Division: Basidiomycota
  • Class: Agaricomycetes
  • Order: Agaricales
  • Family: Strophariaceae
  • Genus: Agrocybe
  • Species: Agrocybe aegerita

Blue Oyster (Pleurotus columbinus) Liquid Culture

Blue Oyster is the cool-weather workhorse, the species most cultivators start with in temperate climates because it performs at the temperatures that naturally occur in basements and outbuildings in fall and spring. Reliable, productive, and forgiving. We include it here because it anchors the cold weather collection with proven, consistent performance.

Blue Oyster — Out-Grow Lab Notes

Parameter Out-Grow Notes
Culture medium LME + dextrose, 2% + 2% in distilled water
Fruiting temp 55 to 72°F
Reliability Our most reliable species in cool growing conditions
Inoculation rate 2 to 3cc per quart of sterilized grain or hardwood substrate
Storage life 6 or more months at normal room temperature

Blue Oyster Taxonomy

  • Kingdom: Fungi
  • Division: Basidiomycota
  • Class: Agaricomycetes
  • Order: Agaricales
  • Family: Pleurotaceae
  • Genus: Pleurotus
  • Species: Pleurotus columbinus

Chestnut Mushroom (Pholiota adiposa) Liquid Culture

Chestnut Mushroom prefers 55 to 68°F for fruiting, which fits squarely in the cold weather range. The caramel-brown caps cluster densely and the mild nutty flavor is genuinely good. It is a species that does not get the attention it deserves, and cool-space cultivators who try it typically stick with it.

Chestnut Mushroom — Out-Grow Lab Notes

Parameter Out-Grow Notes
Culture medium LME + dextrose, 2% + 2% in distilled water
Fruiting temp 55 to 68°F — well suited to cool growing environments
Growth habit Cluster-forming; dense caramel-brown fruiting bodies
Inoculation rate 2 to 3cc per quart of sterilized grain or hardwood substrate
Storage life 6 or more months at normal room temperature

Chestnut Mushroom Taxonomy

  • Kingdom: Fungi
  • Division: Basidiomycota
  • Class: Agaricomycetes
  • Order: Agaricales
  • Family: Strophariaceae
  • Genus: Pholiota
  • Species: Pholiota adiposa

Cold Weather Liquid Culture 5 Pack — Cultivation Overview

Every species in the Cold Weather Mushroom Cultures 5 Pack fruits in the 45 to 72°F range, making this the pack for cultivators who grow in naturally cool spaces without climate control. All five colonize supplemented hardwood sawdust with standard grain spawn methodology. All syringes ship from our McConnell, Illinois lab ready to inoculate.

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