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Description

Black Truffle Mushroom Plate Culture

Black Truffle (Tuber melanosporum) Mushroom Plate Culture.

Each culture plate is a 100x15mm petri dish inoculated with fresh Black Truffle (Tuber melanosporum) mycelium growing on malt extract agar (MEA), and will arrive actively colonizing.

Black Truffle Mushroom Plate Culture Lab Notes

At Out-Grow's mycology lab, we have observed the following traits of this mushroom plate culture:

Black Truffle (Tuber melanosporum) mycelium appears white to cream on culture media, with a thin, appressed texture and limited aerial hyphae. Growth is slow and radial, with colonies remaining relatively small — expect several weeks (20-30+ days) to substantially colonize a 100mm plate at an optimal temperature of 68–77°F. Note: balanced MEA or PDA at approximately pH 6 works well; avoid overly rich or high-glucose media which inhibit growth. Plate behavior does not predict truffle production, as fruiting requires host root inoculation.

Mushroom Plate Culture Use

Mushroom plate cultures, typically on MEA culture plates, are used to grow and preserve mycelium. In most cases, sterile transfers of agar wedges from this plate are used to inoculate fresh culture media or sterilized grain spawn.

Mushroom Plate Culture Storage

Your mushroom plate culture can be wrapped in parafilm and stored at normal room temps, in a sealed baggie, for up to 6 months. Out-Grow recommends you replate your mushroom plate culture every 6 months to ensure longevity of your purchase.

Transfer your Black Truffle mycelium to fresh MEA every 3–6 months to keep your culture healthy and vigorous.

Mushroom Plate Cultures Are Made to Order

All mushroom culture plates are made to order. Once your order is received, Out-Grow will inoculate your plate within 1-3 days. Your mushroom plate culture will then be incubated in our mycology lab for approximately 7-14 days, or until clean healthy mycelium has been verified by our mycologist. Once verified, your order will be shipped promptly. Please allow an additional week for your mushroom plate culture to fully colonize upon arrival, as colonization time varies by species.