Description
About This Mushroom Liquid Culture
Our Shiitake 5 Pack is a premium mushroom liquid culture: live, lab-verified mycelium suspended in sterile nutrient broth and ready to inoculate your grow medium. Each set ships with sterile needles for clean, reliable transfers.
Shiitake Liquid Culture 5 Pack — Product Summary
- Five 10–12cc liquid culture syringes
- Includes: Shiitake Warm Weather (Lentinula edodes), Shiitake Cold Weather (Lentinula edodes), Australian Shiitake (Lentinula lateritia), New Zealand Shiitake (Lentinula novae-zelandiae), Lentinula boryana
- Each syringe contains viable mycelium in sterile nutrient broth
- Store liquid culture syringes at normal room temperature in the original packaging for 6 or more months.
- Ships with sterile needles
Shiitake Liquid Culture 5 Pack — Pack Overview
The Shiitake Mushroom Cultures 5 Pack is the most comprehensive Lentinula collection available anywhere in liquid culture format. Most vendors sell one shiitake strain, maybe two if they are thorough. We carry the full genus including three species that almost nobody else has in their catalog. The Australian, New Zealand, and South American Lentinula species are genuine rarities in the cultivation market, distinct from edodes in genetics, substrate preferences, and flavor. This pack is for cultivators who want to move past standard shiitake and work with the rest of the genus.
Shiitake Liquid Culture 5 Pack — Included Species
Shiitake Warm Weather 55-85°F (Lentinula edodes) Liquid Culture
The warm-range shiitake strain that covers the widest temperature window we offer, 55 to 85°F covers most indoor cultivation spaces without climate control. Standard substrate is supplemented hardwood sawdust or masters mix. Cold shocking the colonized block initiates pinning. This is the strain we recommend for most cultivators starting with shiitake and the one we have sold more of than any other Lentinula in our catalog.
Shiitake Warm Weather 55-85°F — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Fruiting temp | 55 to 85°F — widest range of any shiitake strain we carry |
| Substrate | Supplemented hardwood sawdust; masters mix; oak logs |
| Pinning trigger | Cold shock or temperature drop |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Shiitake Warm Weather 55-85°F Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula edodes
Shiitake Cold Weather 40-60°F (Lentinula edodes) Liquid Culture
The cold-weather shiitake strain selected for performance in cool growing environments, basements, outbuildings, and unheated spaces in autumn and winter. At 40 to 60°F fruiting it fills a temperature window that the warm-weather strain finds challenging. Incubation still runs warmer, but fruiting happens reliably at temperatures where other strains stall. We have had customers successfully fruit this strain in barely-heated spaces through northern winters.
Shiitake Cold Weather 40-60°F — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Fruiting temp | 40 to 60°F — selected specifically for cold-space cultivation |
| Best for | Unheated basements, outbuildings, autumn and winter grows |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Shiitake Cold Weather 40-60°F Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula edodes
Australian Shiitake (Lentinula lateritia) Liquid Culture
Lentinula lateritia is the Australian shiitake relative, a distinct species from edodes that grows on native eucalyptus and other hardwoods in Australian forests. The flavor profile is similar to shiitake but not identical, with slightly more earthiness. It has attracted interest from researchers studying Lentinula genetics and from cultivators who want access to the full genus. We have maintained this culture in our collection for years and it colonizes hardwood substrate reliably.
Australian Shiitake — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Substrate | Hardwood sawdust; eucalyptus-based substrate performs particularly well |
| Species note | Distinct from L. edodes — Australian wild-type genetics |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Australian Shiitake Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula lateritia
New Zealand Shiitake (Lentinula novae-zelandiae) Liquid Culture
Lentinula novae-zelandiae is the New Zealand shiitake, another distinct Southern Hemisphere species confirmed separate from edodes by molecular work. It grows on native New Zealand hardwoods and is rarely available in liquid culture format outside of research contexts. We carry it because our Lentinula collection is built around completeness, and because serious shiitake cultivators and researchers should have access to Southern Hemisphere genetics alongside the standard Asian cultivars.
New Zealand Shiitake — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Rarity | Rarely available in liquid culture format outside research contexts |
| Species note | Southern Hemisphere wild-type distinct from L. edodes by molecular analysis |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
New Zealand Shiitake Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula novae-zelandiae
Lentinula boryana Liquid Culture
Lentinula boryana is the South American shiitake relative, native to forests in Brazil and neighboring countries. It is one of the least commonly cultivated Lentinula species globally and almost impossible to find as liquid culture in the US market. For cultivators and researchers building a complete Lentinula collection, boryana is the piece that is genuinely difficult to source anywhere else. We have maintained it in our collection because the genus deserves representation beyond the single commercially dominant species.
Lentinula boryana — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Rarity | South American species — among the rarest Lentinula cultures in the US market |
| Best for | Research, Lentinula breeding programs, complete genus collections |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Lentinula boryana Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula boryana
Shiitake Liquid Culture 5 Pack — Cultivation Overview
The two Lentinula edodes strains follow standard shiitake cultivation methodology on supplemented hardwood sawdust. The three wild-type Southern Hemisphere species are best approached as research and agar cultures with substrate trials on hardwood sawdust following the same general edodes protocol. All syringes ship from our McConnell, Illinois lab ready to inoculate..
Related Liquid Cultures & Collections
Shiitake Liquid Culture 5 Pack | Mushroom Liquid Culture
Description
About This Mushroom Liquid Culture
Our Shiitake 5 Pack is a premium mushroom liquid culture: live, lab-verified mycelium suspended in sterile nutrient broth and ready to inoculate your grow medium. Each set ships with sterile needles for clean, reliable transfers.
Shiitake Liquid Culture 5 Pack — Product Summary
- Five 10–12cc liquid culture syringes
- Includes: Shiitake Warm Weather (Lentinula edodes), Shiitake Cold Weather (Lentinula edodes), Australian Shiitake (Lentinula lateritia), New Zealand Shiitake (Lentinula novae-zelandiae), Lentinula boryana
- Each syringe contains viable mycelium in sterile nutrient broth
- Store liquid culture syringes at normal room temperature in the original packaging for 6 or more months.
- Ships with sterile needles
Shiitake Liquid Culture 5 Pack — Pack Overview
The Shiitake Mushroom Cultures 5 Pack is the most comprehensive Lentinula collection available anywhere in liquid culture format. Most vendors sell one shiitake strain, maybe two if they are thorough. We carry the full genus including three species that almost nobody else has in their catalog. The Australian, New Zealand, and South American Lentinula species are genuine rarities in the cultivation market, distinct from edodes in genetics, substrate preferences, and flavor. This pack is for cultivators who want to move past standard shiitake and work with the rest of the genus.
Shiitake Liquid Culture 5 Pack — Included Species
Shiitake Warm Weather 55-85°F (Lentinula edodes) Liquid Culture
The warm-range shiitake strain that covers the widest temperature window we offer, 55 to 85°F covers most indoor cultivation spaces without climate control. Standard substrate is supplemented hardwood sawdust or masters mix. Cold shocking the colonized block initiates pinning. This is the strain we recommend for most cultivators starting with shiitake and the one we have sold more of than any other Lentinula in our catalog.
Shiitake Warm Weather 55-85°F — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Fruiting temp | 55 to 85°F — widest range of any shiitake strain we carry |
| Substrate | Supplemented hardwood sawdust; masters mix; oak logs |
| Pinning trigger | Cold shock or temperature drop |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Shiitake Warm Weather 55-85°F Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula edodes
Shiitake Cold Weather 40-60°F (Lentinula edodes) Liquid Culture
The cold-weather shiitake strain selected for performance in cool growing environments, basements, outbuildings, and unheated spaces in autumn and winter. At 40 to 60°F fruiting it fills a temperature window that the warm-weather strain finds challenging. Incubation still runs warmer, but fruiting happens reliably at temperatures where other strains stall. We have had customers successfully fruit this strain in barely-heated spaces through northern winters.
Shiitake Cold Weather 40-60°F — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Fruiting temp | 40 to 60°F — selected specifically for cold-space cultivation |
| Best for | Unheated basements, outbuildings, autumn and winter grows |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Shiitake Cold Weather 40-60°F Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula edodes
Australian Shiitake (Lentinula lateritia) Liquid Culture
Lentinula lateritia is the Australian shiitake relative, a distinct species from edodes that grows on native eucalyptus and other hardwoods in Australian forests. The flavor profile is similar to shiitake but not identical, with slightly more earthiness. It has attracted interest from researchers studying Lentinula genetics and from cultivators who want access to the full genus. We have maintained this culture in our collection for years and it colonizes hardwood substrate reliably.
Australian Shiitake — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Substrate | Hardwood sawdust; eucalyptus-based substrate performs particularly well |
| Species note | Distinct from L. edodes — Australian wild-type genetics |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Australian Shiitake Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula lateritia
New Zealand Shiitake (Lentinula novae-zelandiae) Liquid Culture
Lentinula novae-zelandiae is the New Zealand shiitake, another distinct Southern Hemisphere species confirmed separate from edodes by molecular work. It grows on native New Zealand hardwoods and is rarely available in liquid culture format outside of research contexts. We carry it because our Lentinula collection is built around completeness, and because serious shiitake cultivators and researchers should have access to Southern Hemisphere genetics alongside the standard Asian cultivars.
New Zealand Shiitake — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Rarity | Rarely available in liquid culture format outside research contexts |
| Species note | Southern Hemisphere wild-type distinct from L. edodes by molecular analysis |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
New Zealand Shiitake Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula novae-zelandiae
Lentinula boryana Liquid Culture
Lentinula boryana is the South American shiitake relative, native to forests in Brazil and neighboring countries. It is one of the least commonly cultivated Lentinula species globally and almost impossible to find as liquid culture in the US market. For cultivators and researchers building a complete Lentinula collection, boryana is the piece that is genuinely difficult to source anywhere else. We have maintained it in our collection because the genus deserves representation beyond the single commercially dominant species.
Lentinula boryana — Out-Grow Lab Notes
| Parameter | Out-Grow Notes |
| Culture medium | LME + dextrose, 2% + 2% in distilled water |
| Rarity | South American species — among the rarest Lentinula cultures in the US market |
| Best for | Research, Lentinula breeding programs, complete genus collections |
| Inoculation rate | 2 to 3cc per quart of sterilized grain or hardwood substrate |
| Storage life | 6 or more months at normal room temperature |
Lentinula boryana Taxonomy
- Kingdom: Fungi
- Division: Basidiomycota
- Class: Agaricomycetes
- Order: Agaricales
- Family: Omphalotaceae
- Genus: Lentinula
- Species: Lentinula boryana
Shiitake Liquid Culture 5 Pack — Cultivation Overview
The two Lentinula edodes strains follow standard shiitake cultivation methodology on supplemented hardwood sawdust. The three wild-type Southern Hemisphere species are best approached as research and agar cultures with substrate trials on hardwood sawdust following the same general edodes protocol. All syringes ship from our McConnell, Illinois lab ready to inoculate..